Genome Report | Genome Report
Microbiol. Biotechnol. Lett. 2023; 51(3): 293-295
https://doi.org/10.48022/mbl.2308.08006
Gi Yong Lee and Soo-Jin Yang*
Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea
Correspondence to :
Soo-Jin Yang, soojinjj@snu.ac.kr
Linezolid, the first oxazolidinone introduced into human clinical use, has become a last resort antibiotic in treatment of serious infections caused by Gram-positive pathogens, including methicillin-resistant staphylococci and vancomycin-resistant enterococci. Although oxazolidinones are strictly prohibited for use in food-producing animals, occurrence of linezolid-resistant staphylococci has recently been reported in livestock farms in Korea. Here, we report the complete genome sequence of an optrA-positive linezolid-resistant Staphylococcus rostri strain PJFA-333 isolated from a pig farm in Korea.
Keywords: Staphylococcus rostri, linezolid resistance, optrA
Emergence of linezolid resistance in
A linezolid-resistant
Table 1 . Genetic characteristics of the linezolid-resistant
Genetic features | PJFA-333 |
Source | Pig |
Antimicrobial resistancea | CHL-CLI-LZD-TET |
Genome size | 2,390,967 bp |
GC content | 38.4% |
No. of contigs (bp, G+C contet, and CDS) | 7 |
Chromosome | 2,337,213 bp; 38.5%; 2,239 |
Plasmids | pSR7-1 (36,227 bp; 32.8%; 40) |
pSR7-2 (8,025 bp; 32.4%; 9) | |
pSR7-3 (4,047 bp; 29.1%; 4) | |
pSR7-4 (2,706 bp; 27.2%; 3) | |
pSR7-5 (1,491 bp; 32.9%; 2) | |
pSR7-6 (1,258 bp; 33.8%; 2) | |
No. of RNAs | 76 (tRNA, 57 ; and rRNA, 19) |
Antimicrobial resistance genes (location) | |
GenBank accession numbers | CP113107 (Chr) |
pSR7-1 (CP113108), pSR7-2 (CP113109), pSR7-3 (CP113110), pSR7-4 (CP113111), pSR7-5 (CP113112), pSR7-6 (CP113113) |
aCHL, chloramphenicol; CLI, clindamycin; LZD, linezolid; and TET, tetracycline
CDS, coding sequence; chr, chromosome
The complete genome sequences of the PJFA-333 strain (CP113107) and the six plasmids (CP113108 - CP113113) have been deposited in the GenBank sequence database.
This study was supported by grants from Research of Korea Centers for Disease Control and Prevention (2020ER540500) and Ministry of Food and Drug Safety (23194MFDS012).
The authors have no financial conflicts of interest to declare.
Jun Bong Lee, Nguyen Thi Mai Tho, Se Kye Kim, and Jang Won Yoon
Microbiol. Biotechnol. Lett. 2024; 52(1): 88-90 https://doi.org/10.48022/mbl.2402.02004