Article Search

Microbiology and Biotechnology Letters


View PDF

Bioactive Compounds / Food Microbiology  |  Bioactive compounds and Physiological properties

Microbiol. Biotechnol. Lett. 2020; 48(3): 303-309

Received: January 30, 2020; Accepted: March 11, 2020

유산균으로 발효한 침향공진단으로부터 분리한 Nodakenetin의 Neuraminidase 활성 억제 효능

Neuraminidase-inhibition Activity of Nodakenetin from Gongjin-dan Fermented by Lactic Acid Bacteria

Ji Hyun Seo , Dong Jun Park , So Young Lee , Ho Song Cho and Mu Hyun Jin *

LG Science Research Park, LG Household and Healthcare Ltd.

The purpose of this study was to identify the changes in the components of unfermented Gongjin-dan (GD) and fermented Gongjin-dan (FGD) and to confirm whether GD or FGD has an inhibitory effect on viral neuraminidase (NA) activity. A major component of FGD was isolated and identified as nodakenetin, which is the aglycone of nodakenin. After fermentation, the nodakenetin content in FGD was approximately 10- fold higher than that in GD. Then, we examined the viral NA-inhibitory activity of GD, FGD, nodakenin, and nodakenetin. At a concentration of 500 μg/ml, FGD inhibited viral NA activity by 92% compared to the DMSO-treated control, while GD barely inhibited viral NA activity. In addition, 250 μg/ml of nodakenetin inhibited viral NA activity by 68% compared to the control, while nodakenin inhibited viral NA activity by only 4% at the same concentration as nodakenetin. Collectively, these results suggest that FGD has a more remarkable viral NA-inhibitory activity than GD because the content of the anti-viral component nodakenetin was higher in FGD due to the hydrolysis of nodakenin by Lactobacillus plantarum KCTC 3104.

Keywords: Gongjin-dan, fermentation, Lactobacillus plantarum, nodakenin, nodakenetin, neuraminidase

  1. Wi YL. 1990. Seuideughyobang, pp. 271. Uiseongdang, Seoul.
  2. Heo J. 1993. Hangul Donguibogam. pp. 817. Minjoong-Seogak,Seoul.
  3. Kim HJ, Hwang SY, Mok JY, Hwang BS, Jeong SI, Jang SI. 2009. Gagam-Gongjindan extract attenuates immune responses to ovalbumin in Balb/c mice. Kor. J. Herbology 24: 127-135.
  4. Hwang DY. 2014. Daeyeog Jeungmaeg·Bangyaghabpyeon. pp. 163-164. Namsandang, Seoul.
  5. Lee KH, Choi DK. 2016. Study on classification system and use of song dynasty Agarwood Line. Chin. Hist. Res. 100: 171-203.
  6. You YH, Koh JH, Chung SO, Jun WJ, Kim KM. 2009. Effects of fermented Ssanghwatang on swimming capacity in mice. Food. Sci. Biotechnol. 18: 275-277.
  7. Kerry RG, Patra JK, Gouda S, Park YH, Shin HS, Das G. 2018. Benefaction of probiotics for human health: A review. J. Food Drug Anal. 26: 927-939.
    Pubmed CrossRef
  8. Suez J, Zmora N, Segal E, Elinav E. 2019. The pros, cons, and many unknowns of probiotics. Nat. Med. 25: 716-729.
    Pubmed CrossRef
  9. Knackstedt R, Knackstedt T, Gatherwright J. 2019. The role of topical probiotics in skin conditions: A systematic review of animal and human studies and implications for future therapies. Exp. Dermatol. 29: 15-21.
    Pubmed CrossRef
  10. Siezen RJ, Tzeneva VA, Castioni A, Wels M, Phan H, Rademaker J, et al. 2010. Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches. Environ. Microbiol. 12: 758-773.
    Pubmed CrossRef
  11. Herbel SR, Vahjen W, Wieler LH, Guenther S. 2013. Timely approaches to identify probiotic species of the genus Lactobacillus. Gut Pathog. 5: 27.
    Pubmed KoreaMed CrossRef
  12. Sun Z, Harris H, McCann A, Guo C, Argimon S, Zhang W, et al. 2015. Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera. Nat. Commun. 6: 8322.
    Pubmed KoreaMed CrossRef
  13. Arasu MV, Kim DH, Kim PI, Jung MW, Ilavenil S, Jane M, et al. 2014. In vitro antifungal, probiotic and antioxidant properties of novel Lactobacillus plantarum K46 isolated from fermented sesame leaf. Ann. Microbiol. 64: 1333-1346.
  14. Murofushi Y, Villena J, Morie K, Kanmani P, Tohno M, Shimazu T, et al. 2015. The toll-like receptor family protein P105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14O. Mol. Immunol. 64:63-75.
    Pubmed CrossRef
  15. Wang K, Li W, Rui X, Chen X, Jiang M, Dong M. 2014. Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 70810. Int. J. Biol. Macromol. 63:133-139.
    Pubmed CrossRef
  16. Woo JY, Gu W, Kim KA, Jang SE, Han MJ, Kim DH. 2014. Lactobacillus pentosus var. plantarum C29 ameliorates memory impairment and inflammaging in a D-galactose-induced accelerated aging mouse model. Anaerobe 27: 22-26.
    Pubmed CrossRef
  17. Jung YJ, Han DO, Choi BH, Park C, Lee HJ, Kim SH, et al. 2007. Effect of fermented herbal extracts, HP-1 on enzyme activities and gene expressions related to alcohol metabolism in ethanolloaded rats. Korean J. Oriental Physiol. Pathol. 21: 387-391.
  18. Zhang P, Yang XW. 2009. Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method. J. Asian Nat. Prod. Res. 11: 371-379.
    Pubmed CrossRef
  19. Kwang JL, Song NY, Roh JH, Liang C, Ma JY. 2013. Analysis of bioconversed components in fermented Jaeumganghwa-tang by Lactobacillus. J. Appl. Biol. Chem. 56: 131-135.
  20. Yang MC, Jeong SW, Ma JY. 2011. Analysis of constituents in Sipjundaebotangs fermented by lactic acid bacteria. Korean J. Microbiol. Biotechnol. 39: 350-356.
  21. Kim DS, Roh JH, Cho CW, Ma JY. 2012. Analysis of nodakenetin from Samultangs fermented by lactose bacteria strains. Kor. J. Herbology 27: 35-39.
  22. Lee KW, Cho HK, Yoo HR, Seol IC, Kim YS. 2018. The effects of an extract of fermented Artemisiae Iwayomogii Herba, Curcumae longae, Crataegi fructus and Salviae miltiorrhizae radix on antiinflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC Cells. J. Int. Korean Med. 39: 480-494.
  23. Kang DH, Kim HS. 2011. Functionality analysis of korean medicine fermented by Lactobacillus strains. Korean J. Microbiol. Biotechnol.39: 259-265.
  24. Lee JH, Jo DC, Kim CG, Moon SJ, Park TY, Ko YS. et al. 2013. A literature review of effectiveness on the Gongjin-dan (Gongchendan). J. Korean Med. Rehabi. 23: 69-78.
  25. Moscona A. 2005. Neuraminidase Inhibitors for Influenza. N. Engl. J. Med. 353: 1363-1373.
    Pubmed CrossRef
  26. Liu AL, Wang HD, Lee MY, Wang YT, Du GH. 2008. Structure–activity relationship of flavonoids as influenza virus neuraminidase inhibitors and their in vitro anti-viral activities. Bioorg. Med. Chem. 16: 1741-1747.
    Pubmed CrossRef
  27. Kim EH, Lee JH, Song MS, Baek YH, Choi YK, Ma JY. 2012. Administration of novel oriental medicine, KIOM-C, protect against influenza virus. J. Biomed. Res. 13: 149-156.
  28. Lee SH, Shin DS, Kim JS, Oh KB, Kang SS. 2003. Antibacterial coumarins from Angelica gigas roots. Arch. Pharm. Res. 26: 449-452.
    Pubmed CrossRef
  29. Seong GU, Beak ME, Lee YJ, Won JH. 2018. Quality evaluation of Angelica gigas Nakai with different drying methods and different root parts. Kor. J. Herbology 33: 85-91.
  30. Landete JM, Curiel JA, Rodrıguez H, Rivas B, Munoz R. 2014. Aryl glycosidases from Lactobacillus plantarum increase antioxidant activity of phenolic compounds. J. Funct. Foods. 7: 322-329.
  31. Kim DH, Kim DY, Kim YC, Jung JW, Lee S, Yoon BH, et al. 2007. Nodakenin, a coumarin compound, ameliorates scopolamineinduced memory disruption in mice. Life Sci. 80: 1944-1950.
    Pubmed CrossRef
  32. Gao Q, Jeon SJ, Jung HA, Lee HE, Park SJ, Lee YH, et al. 2015. Nodakenin enhances cognitive function and adult hippocampal neurogenesis in mice. Neurochem. Res. 40: 1438-1447.
    Pubmed CrossRef
  33. Xiong Y, Wang JS, Yu H, Zhang X, Miao C, Ma S. 2014. The effects of nodakenin on airway inflammation, hyper-responsiveness and remodeling in a murine model of allergic asthma. Immunopharmacol. Immunotoxicol. 36: 341-348.
    Pubmed CrossRef
  34. Hideji T, Yun YS, Hiroshi M, Koich T, Lee SR. 1994. Cytotoxic coumarins from the roots of Angelica gigas Nakai. J. Oriental Bot. Res.7: 13-15.

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.