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Microbiology and Biotechnology Letters

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Environmental Microbiology / Microbial Diversity  |  Environmental Microbiology

Microbiol. Biotechnol. Lett. 2020; 48(1): 72-78

https://doi.org/10.4014/mbl.1909.09004

Received: September 10, 2019; Accepted: October 28, 2019

질산화-탈질 연계공정에서 질소화합물 및 기능성 유전자 거동

Dynamics of Nitrogen Compounds and Functional Genes in a Nitrification- Denitrification Coupling Process

Ji-Hyeon Kwon , Hyung-Joo Park , Yun-Yeong Lee and Kyung Suk Cho *

Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea

The dynamics of nitrogen compounds and RNA-based functional genes were characterized in the nitrification- denitrification coupling process. For the removal of residual ammonium, intermittent aeration was introduced in the denitrification reactor. N2O production was not observed in both reactors. In both reactors, the nitrifying genes (achaeal-amoA, bacterial-amoA and hor) and denitrifying genes (narG, nirK, norB and nosZ) had a copy number of 3.92 × 102 −7.25 × 105 and 2.85 × 102 −3.06 × 104 per ng of DNA, respectively. These results suggest that denitrification and nitrification reactions occur in both the nitrification and denitrification reactors, respectively. Therefore, the coupling process is a promising one for the conversion of ammonium to nitrogen without generating N2O.

Keywords: Nitrification, denitrification, functional genes, wastewater treatment, nitrous oxide, coupling process

  1. Jin P, Chen Y, Xu T, Chi Z, Zheng Z. 2019. Efficient nitrogen removal by simultaneous heterotrophic nitrifying-aerobic denitrifying bacterium in a purification tank bioreactor amended with two-stage dissolved oxygen control. Bioresour. Technol. 281:392-400.
    Pubmed CrossRef
  2. Zanetti L, Frison N, Nota E, Tomizioli M, Bolzonella D, Fatone F. 2012. Progress in real-time control applied to biological nitrogen removal from wastewater. A short-review. Desalination 286: 1-7.
    CrossRef
  3. Antileo C, Werner A, Ciudad G, MunoZC, Bornhardt C, Jeison D, et al. 2006. Novel operational strategy for partial nitrification to nitrite in a sequencing batch rotating disk reactor. Biochem. Eng. J. 32: 69-78.
    CrossRef
  4. Gao J, Duan Y, Liu Y, Zhuang X, Liu Y, Bai Z, et al. 2019. Long-and short-chain AHLs affect AOA and AOB microbial community composition and ammonia oxidation rate in activated sludge. J. Environ. Sci. 78: 53-62.
    Pubmed CrossRef
  5. Wunderlin P, Mohn J, Joss A, Emmenegger L, Siegrist H. 2012. Mechanisms of N2O production in biological wastewater treatment under nitrifying and denitrifying conditions. Water. Res. 46:1027-1037.
    Pubmed CrossRef
  6. Zheng Y, Hou L, Liu M, Newell S, Yin G, Yu C, et al. 2017. Effects of silver nanoparticles on nitrification and associated nitrous oxide production in aquatic environments. Sci. Adv. 3: 1-11.
    Pubmed KoreaMed CrossRef
  7. Zumft WG. 1997. Cell biology and molecular basis of denitrification. Microbiol. Mol. Biol. Rev. 61: 533-616.
    Pubmed KoreaMed CrossRef
  8. Chai H, Xiang Y, Chen R, Shao Z, Gu L, Li L, et al. 2019. Enhanced simultaneous nitrification and denitrif ication in treating low carbon-to-nitrogen ratio wastewater: Treatment performance and nitrogen removal pathway. Bioresour. Technol. 280: 51-58.
    Pubmed CrossRef
  9. Chen Y, He H, Liu H, Li H, Zeng G, Xia X, et al. 2018. Effect of salinity on removal performance and activated sludge characteristics in sequencing batch reactors. Bioresour. Technol. 249: 890-899.
    Pubmed CrossRef
  10. He H, Chen Y, Li X, Cheng Y, Yang C, Zeng G. 2017. Influence of salinity on microorganisms in activated sludge processes: A review. Int. Biodeterior. Biodegrad. 119: 520-527.
    CrossRef
  11. Herrmann E, Young W, Rosendale D, Conrad R, Riedel CU, Egert M. 2017. Determination of resistant starch assimi lating bacteria in fecal samples of mice by in vitro RNA-based stable isotope probing. Front. Microbiol. 8: 1331.
    Pubmed KoreaMed CrossRef
  12. Fan Z, Zeng W, Wang B, Chang S, Peng Y. 2019. Analysis of microbial community in a continuous flow process at gene and transcription level to enhance biological nutrients removal from municipal wastewater. Bioresour. Technol. 286: 121374.
    Pubmed CrossRef
  13. Su Q, Ma C, Domingo-Félez C, Kiil AS, Thamdrup B, Jensen MM, et al. 2017. Low nitrous oxide production through nitrifierdenitrification in intermittent-feed high-rate nitritation reactors. Water. Res. 123: 429-438.
    Pubmed CrossRef
  14. Chen L, Hoff SJ. 2012. A two-stage wood chip-based biofilter system to mitigate odors from a deep-pit swine building. Appl. Eng. Agric. 28: 893-901.
    CrossRef
  15. Lee YY, Choi H, Cho KS. 2019. Effects of carbon source, C/N ratio, nitrate, temperature, and pH on N2O emission and functional denitrifying genes during heterotrophic deni trification. J. Environ. Sci. Heal. - Part A Toxic/Hazardous. Subst. Environ. Eng. 54: 16-29.
    Pubmed CrossRef
  16. Kim TG, Moon KE, Yun J, Cho KS. 2013. Comparison of RNA- and DNA-based bacterial communities in a lab-scale methanedegrading biocover. Appl. Microbiol. Biotechnol. 97: 3171-3181.
    Pubmed CrossRef
  17. Kim TG, Yi T, Lee EH, Ryu HW, Cho KS. 2012. Characterization of a methane-oxidizing biofilm using microarray, and confocal microscopy with image and geostatic analyses. Appl. Microbiol. Biotechnol. 95: 1051-1059.
    Pubmed CrossRef
  18. Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB. 2005. Ubiqity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc. Natl. Acad. Sci. USA 102: 14683-14688.
    Pubmed KoreaMed CrossRef
  19. Rotthauwe JH, Witzel KP, Liesack W. 1997. The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl. Environ. Microbiol. 63: 4704-4712.
    Pubmed KoreaMed CrossRef
  20. Schmid MC, Hooper AB, Klotz MG, Woebken D, Lam P, Kuypers MMM, et al. 2008. Environmental detection of octahaem cytochrome c hydroxylamine/hydrazine oxidoreductase genes of aerobic and anaerobic ammonium-oxidizing bacteria. Environ. Microbiol. 10: 3140-3149.
    Pubmed CrossRef
  21. Schreiber F, Wunderlin P, Udert KM, Wells GF. 2012. Nitric oxide and nitrous oxide turnover in natural and engineered microbial communities: Biological pathways, chemical reactions, and novel technologies. Front. Microbiol. 3: 372.
    Pubmed KoreaMed CrossRef
  22. Bru D, Sarr A, Philippot L. 2007. Relative abundances of proteo bacterial membrane-bound and periplasmic nitrate reductases in selected environments. Appl. Environ. Microbiol. 73: 59715974.
    Pubmed KoreaMed CrossRef
  23. Henry S, Baudoin E, López-Gutiérrez JC, Martin-Laurent F, Brauman A, Philippot L. 2004. Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR. J. Microbiol. Methods 59: 327-335.
    Pubmed CrossRef
  24. Song K, Suenaga T, Hamamoto A, Satou K, Riya S, Hosomi M, et al. 2014. Abundance, transcription levels and phyl ogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant. J. Biosci. Bioeng. 118: 289-297.
    Pubmed CrossRef
  25. Casciotti KL, Ward BB. 2005. Phylogenetic analysis of nitric oxide reductase gene homologues from aerobic ammonia-oxidizing bacteria. FEMS Microbiol. Ecol. 52: 197-205.
    Pubmed CrossRef
  26. Henry S, Bru D, Stres B, Hallet S, Philippot L. 2006. Quantitative detection of the nosZ gene, encoding nitrous oxide reductase, and comparison of the abundances of 16S rRNA, narG, nirK, and nosZ genes in soils. Appl. Environ. Microbiol. 72: 5181-5189.
    Pubmed KoreaMed CrossRef
  27. Li P, Wang S, Peng Y, Liu Y, He J. 2015. The synergistic effects of dissolved oxygen and pH on N2O production in biological domestic wastewater treatment under nitrifying conditions. Environ. Technol. 36: 1623-1631.
    Pubmed CrossRef
  28. Rodriguez-Caballero A, Aymerich I, Poch M, Pijuan M. 2014. Evaluation of process conditions triggering emissions of greenhouse gases from a biological wastewater treatment system. Sci. Total Environ. 493: 384-391.
    Pubmed CrossRef
  29. Zhu X, Chen Y. 2011. Reduction of N2O and NO generation in anaerobic-aerobic (low dissolved oxygen) biological wastewater treatment process by using sludge alkaline fermentation liquid. Environ. Sci. Technol. 45: 2137-2143.
    Pubmed CrossRef
  30. Marin JCA, Caravelli AH, Zaritzky NE. 2019. Performance of anoxic-oxic sequencing batch reactor for nitrification and aerobic denitrification. Biotechnol. Bioeng. 1-22, doi: 10.5772/intechopen. 84775.
  31. Limpiyakorn T, Kurisu F, Sakamoto Y, Yagi O. 2007. Effects of ammonium and nitrite on communities and populations of ammonia-oxidizing bacteria in laboratory-scale continuousflow reactors. FEMS Microbiol. Ecol. 60: 501-512.
    Pubmed CrossRef
  32. Quan ZX, Rhee SK, Zuo JE, Yang Y, Bae JW, Park JR, Lee ST, Park YH. 2008. Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor. Environ. Microbiol. 10: 3130-3139.
    Pubmed CrossRef
  33. Ma B, Peng Y, Zhang S, Wang J, Gan Y, Chang J, Wang S, Wang S, Zhu G. 2013. Performance of anammox UASB reactor treating low strength wastewater under moderate and low temperatures. Bioresour. Technol. 129: 606-611.
    Pubmed CrossRef
  34. Ye L, Zhang T. 2011. Ammonia-oxidizing bacteria dominates over ammonia-oxidizing archaea in a saline nitrification reactor under low DO and high nitrogen loading. Biotechnol. Bioeng. 108: 2544-2552.
    Pubmed CrossRef
  35. Mosier AC, Francis CA. 2008. Relative abundance and diversity of ammonia-oxidizing archaea and bacteria in the San Francisco Bay estuary. Environ. Microbiol. 10: 3002-3016.
    Pubmed CrossRef
  36. Zhi W, Ji G. 2014. Quantitative response relationships between nitrogen transformation rates and nitrogen functional genes in a tidal flow constructed wetland under C/N ratio constraints. Water. Res. 64: 32-41.
    Pubmed CrossRef
  37. Ji G, Zhi W, Tan Y. 2012. Association of nitrogen micro-cycle functional genes in subsurface wastewater infiltration systems. Ecol. Eng. 44: 269-277.
    CrossRef
  38. Poo KM, Im JH, Jun BH, Kim JR, Hwang IS, Choi KS, et al. 2006. Full-cyclic control strategy of SBR for nitrogen removal in strong wastewater using common sensors. Water. Sci. Technol. 53: 151160.
    Pubmed CrossRef
  39. Ji B, Yang K, Zhu L, Jiang Yu, Wang H, Zhou J, et al. 2015. Aerobic denitrification: A review of important advances of the last 30 years. Biotechnol. Bioprocess Eng. 20: 643-651.
    CrossRef

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