Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Bioactive Compounds / Food Microbiology  |  Bioactive compounds and Physiological properties

Microbiol. Biotechnol. Lett. 2019; 47(4): 515-521

https://doi.org/10.4014/mbl.1908.08008

Received: August 20, 2019; Accepted: October 15, 2019

와송이 고지방 식이로 유도된 비만 쥐의 간내 지질 및 항산화 대사에 미치는 영향

Effects of Water Extract from Orostachys japonicus on Lipid and Antioxidant Metabolism in the Liver of Obese Mice

Hyeong-Seon Lee 1 and Soo-Hwan Kim 2*

1Department of Biomedical Laboratory Science, Jungwon University, 2Department of Biomedical Laboratory Science, Gimcheon University

The purpose of this study was to investigate the changes in the liver lipid and antioxidant metabolism in obese rats following ingestion of Orostachys japonicus (OJ) hot water extract. The rats were fed a high fat diet for 8 weeks to induce obesity. In the experimental group, 2.5% or 5% OJ hot water extract was added to the diets for 6 weeks. To determine the changes in the liver lipid and antioxidant metabolism in obese rats, the livers were collected and weighed, lipid content was measured, and antioxidant enzyme activity was determined. A significant decrease in liver weight was observed in the group fed with OJ hot water extracts and decreased lipid accumulation was observed in the morphological observation of liver tissue. Total cholesterol and triglyceride contents were also decreased. Both OJ extracts significantly decreased 2,2-diphenyl-1-picrylhydrazyl radical and intracellular reactive oxygen species levels. Further, the levels of the antioxidant enzymes superoxide dismutase and catalase were significantly increased in rats treated with OJ extracts. These results suggest that the intake of OJ hot water extracts may improve liver lipid metabolism by activating antioxidant enzymes.

Keywords: Orostachys japonicus, lipid metabolism, antioxidant metabolism, obesity induced rats, liver

  1. Lim EJ, Kim MJ, Kim HJ, Lee SH, Jeon BG. 2018. Comparative analysis on anti-aging, anti-adipogenesis, and anti-tumor effects of Green Tea polyphenol epigallocatechin-3-gallate. J. Life Sci. 28: 1201-1211.
  2. Holst B, Williamson G. 2008. Nutrients and phytochemicals: from bioavailability to bioefficacy beyond anti-oxidants. Curr. Opin. Biotechnol. 19: 73-82.
    Pubmed CrossRef
  3. Kim JY, Junh EJ, Won YS, Lee JH, Shin DY, Seo KI. 2012. Cultivated Orostachys japonicus induces apoptosis in human colon cancer cells. Korea J. Food Sci. Technol. 44: 317-323.
    CrossRef
  4. Park HJ, Young HS, Kim JO, Rhee SH, Choi JS. 1991. A study on the chemical constituents of Orostachys japonicus A. Berger. Korean J. Pharmacogn. 22: 78-84.
  5. Park JG, Park JC, Hur JM, Park SJ, Choi DR, Shin DY, et al. 2000. Phenolic compounds from Orostachys japonicus having antiHIV-1 protease activity. Nat. Prod. Sci. 6: 117-121.
  6. Kwon J, Han KS. 2004. Effects of Orostachys japonicus A. Berger on the immune system. Korean J. Medicinal Crop Sci. 12: 315-320.
  7. Lee HS, Lee GS, Kim SH, Kim HK, Suk DH, Lee DS. 2014. Antioxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway. BMB Rep. 47: 98-103.
    Pubmed KoreaMed CrossRef
  8. Yoon SY, Lee SY, Kim KBWR, Song EJ, Kim SJ, Lee SJ, et al. 2009. Antimicrobial activity of the solvent extract from different parts of Orostachys japonicus. J. Korean Soc. Food Sci. Nutr. 38: 14-18.
    CrossRef
  9. Kim SG, Choi JW, Park HJ, Lee SM, Jung HJ. 2009. Anti-hyperlipidemic effects of the flavonoid-rich fraction from the methanol extrect of Orostachy japonicus in rats. Korean J. Pharmacogn. 40: 51-58.
  10. Zhang GF. 2008. Anti-diabetic potential of Orostachys japonicus in streptozotocin induced diabetes mellitus rats. MS Thesis. Gyeongsang National University, Jinju, Korea.
  11. Ryu DS, Lee HS, Lee GS, Lee DS. 2012. Effects of the ethylacetate extract of Orostachys japonicus on induction of apoptosis through the p53-mediated signaling pathway in human gastric cancer cells. Biol. Pharm. Bull. 35: 660-665.
    Pubmed CrossRef
  12. Lee HS, Ryu DS, Lee GS, Lee DS. 2012. Anti-inflammatory effects of dichloromethane fraction from Orostachys japonicus in RAW 264.7 cells: suppression of NF-κB activation and MAPK signaling. J. Ethnopharmacol. 140: 271-276.
    Pubmed CrossRef
  13. Kim JE, Bae SM, Nam YR, Bae EY, Ly SY. 2019. Antioxidant activity of ethanol of Lycium barbarum’s leaf with removal of chlorophyll. J. Nutr. Health. 52: 26-35.
    CrossRef
  14. Lee SM, Park MY, Kim OK, Lee JM, Jun WJ. 2016. Anti-obesity effect of Eriobatrya japonica leaves extract on obese mice induced by high-fat diet. J. Korean Soc. Food Sci. Nutr. 45: 1202-1206.
    CrossRef
  15. Lee JY, Park JY, Kim HD, Lee SE, Lee JH, Lee YJ, et al. 2019. AntiOxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena. J. Nutr. Health 52: 250-257.
    CrossRef
  16. Lee SJ, Song EJ, Lee SY, Kim KBWR, Kim SJ, Yoon SY, et al. 2009. Antioxidant activity of leaf, stem and root extracts from Orostachys japonicus and their heat and pH stabilities. J. Korean Soc. Food Sci. Nutr. 38: 1571-1579.
    CrossRef
  17. Lim SM, Park HJ, Cho YJ. 2017. Antioxidative and biological activity of extracts from Orostachys japonicus. J. Appl. Biol. Chem. 60: 293-300.
    CrossRef
  18. Lee HJ, Kim HS, Seo SW. 2017. Anti-obestity effect of Aster Yomena ethanol extract in high fat diet-induced obes mice. J. Physiol. Pathol. Korean Med. 31: 348-355.
    CrossRef
  19. Alara OR, Abdurahman NH, Mudalip SK, Olalere OA. 2018. Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves. J. Food Meas Charact. 12: 311-316.
    CrossRef
  20. Lee YH, Ho JN, Dong MS, Park JH, Kim HK, Hong BS, et al. 2005. Transfected HepG2 cells for evaluation of catechin effects on alcohol-induced CYP2E1 cytotoxicity. J. Microbiol. Biotechnol. 15: 1310-1316.
  21. Asayama K, Nakane T, Dobashi K, Kodera K, Hayashibe H, Uchida N, et al. 2001. Effect of obesity and troglitazone on expression of two glutathione peroxidases: cellular and extracellular types in serum, kidney and adipose tissue. Free Radic Res. 34: 337-347.
    Pubmed CrossRef
  22. Shyamala MP, Venukumar MR, Latha MS. 2003. Antioxidant potential of the syzygium aromaticum (GAERTN.) Linn. (Cloves) in rats fed with high fat diet. Indian J. Pharmacol. 35: 99-103.
  23. Lee HS, Li L, Kim HK, Bilehal D, Li W, Lee DS, et al. 2010. The protective effects of Curcuma longa Linn. extract on carbon tetrachloride-induced hepatotoxicity in rats via upregulation of nrf2. J. Microbiol. Biotechnol. 20: 1331-1338.
    Pubmed CrossRef
  24. Kim HK, Li L, Lee HS, Park MO, Bilehal D, Li W, et al. 2009. Protective effects of Chlorella vulgaris extract on carbon tetrachlorideinduced acute liver injury in mice. Food Sci. Biotechnol. 5: 1186-1192.

Starts of Metrics

Share this article on :

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.