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Microbiology and Biotechnology Letters

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Environmental Microbiology / Microbial Diversity  |  Agricultural Microbiology

Microbiol. Biotechnol. Lett. 2019; 47(3): 434-440

https://doi.org/10.4014/mbl.1901.01002

Received: January 7, 2019; Accepted: February 15, 2019

김치에서 분리된 젖산균의 β-glucuronidase 활성 탐색

Exploration of β-Glucuronidase Activity of Lactic Acid Bacteria Isolated from Kimchi

In-Ung Shin 1, Se-Young Kwun 1, Eun-Hee Park 1, Jae-Hyoung Yi 2 and Myoung-Dong Kim 1*

1Division of Food Biotechnology and Biosystems Engineering, Kangwon National University, 2Ginseng and Medicinal Plant Research Institute, Gangwon Agricultural Research & Extension Services

Abstract

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Lactic acid bacteria (LAB) isolated from kimchi were studied for their β-glucuronidase activity. Among the 156 strains tested, 52 strains utilized glucuronic acid as a carbon source and their intracellular β-glucuronidase activities were significantly higher than their extracellular activities. Leuconostoc mesenteroides KFRI 73007 isolated from turnip kimchi exhibited the highest intracellular β-glucuronidase activity of 0.77 ± 0.01 U/mg protein, which was further increased to 1.14 ± 0.01 U/mg protein under optimized reaction conditions (pH 7, 37℃). The activity of β-glucuronidase was notably decreased by the addition of divalent cations, and glucuronic acid was the best carbon source to produce β-glucuronidase in Leu. mesenteroides KFRI 73007.

Keywords: β-Glucuronidase, Kimchi, Lactic acid bacteria, Leuconostoc mesenteroides

References

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