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Microbiology and Biotechnology Letters

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Environmental Microbiology / Microbial Diversity  |  Agricultural Microbiology

Microbiol. Biotechnol. Lett. 2019; 47(3): 390-400

https://doi.org/10.4014/mbl.1904.04002

Received: April 1, 2019; Accepted: April 29, 2019

환경스트레스와 관련된 indole-3-acetic acid 및 1-aminocylopropane-1-carboxylyic acid deaminase 활성을 갖는 박테리아의 분리와 특성 연구

Isolation and Characterization of Indole-3-acetic acid- and 1-aminocylopropane-1-carboxylyic Acid Deaminase-producing Bacteria Related to Environmental Stress

Hee Sook Kim 1, Ji-Youn Kim 1, Song Min Lee 1, Hye-Jung Park 1, Sang-Hyeon Lee 2, Jeong Su Jang 1* and Mun Hyon Lee 1

1Food Research Center, Angel Co., Ltd., Republic of Korea, 2Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University, Republic of Korea

Abstract

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In this study, strains isolated from soil samples collected from Busan, Changwon, and Jeju Island were examined to verify their abilities of phosphate solubilization and nitrogen fixation, production of indole-3- acetic acid (IAA), siderophore, and 1-aminocylopropane-1-carboxylyic acid (ACC) deaminase in order to select strains that promote plant growth and play a role in biocontrol of pests or pathogens. According to the results of this study, most of the isolated strains were found to have ability of phosphate solubilization, nitrogen fixation, IAA production, siderophore production, and production of ACC deaminase. These isolated strains might help plant growth by directly improving absorption of nutrients essential for phosphate solubilization and nitrogen fixation. In addition, they can promote plant growth and control resistance to plant diseases through extracellular enzyme activity and antifungal activity. In addition, most of the selected strains were found to survive in various environmental conditions such as temperature, salinity, and pH. Therefore, Pseudomonas plecoglossicida ANG14, Pseudarthrobacter equi ANG28, Beijerinckia fluminensis ANG34, and Acinetobacter calcoaceticus ANG35 were finally selected through a comparative advantage analysis to suggest their potential as novel biological agents. Further studies are necessary in order to prove their efficacy as novel biological agents through formulation and optimization of effective microorganisms, their preservation period, and crop cultivation tests.

Keywords: 1-aminocyclopropane-1-carboxylic acid deaminase, indole acetic acid, phosphate solubilization, siderophores

References

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