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Microbiology and Biotechnology Letters

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Bioactive Compounds / Food Microbiology  |  Food Biotechnology

Microbiol. Biotechnol. Lett. 2019; 47(3): 343-349

https://doi.org/10.4014/mbl.1905.05002

Received: May 3, 2019; Accepted: June 10, 2019

Some Properties and Microbial Community Changes of Gul (Oyster) Jeotgal during Fermentation

Jeong A Kim 1, Zhuang Yao 1, Hyun-Jin Kim 1, 2 and Jeong Hwan Kim 1, 2*

1Division of Applied Life Science (BK21 plus), Graduate School, 2Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea

Abstract

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Gul (oyster) jeotgals (GJs) were prepared with solar salt aged for 3 years. One sample was fermented with starters, Bacillus subtilis JS2 and Tetragenococcus halophilus BS2-36 (each 106 CFU/g), and another one without starters for 49 days at 10℃. The initial bacilli and lactic acid bacteria (LAB) counts of non-starter GJ were 3.20 x 102 and 7.67 x 101 CFU/g at day 0, and increased to 1.37 x 103 and 1.64 x 106 CFU/g at day 49. Those of starter GJ were 2.10 x 105 and 3.30 x 107 CFU/g at day 49, indicating growth of starters. The pH of GJ were 5.93 ± 0.01 (non-starter) and 5.92 ± 0.01 (starter) at day 0 and decreased to 5.78 ± 0.01 (non-starter) and 5.75 ± 0.01 (starter) at day 49. Amino-type nitrogen (ANN) increased continuously during fermentation, and 407.19 ± 15.85 (non-starter) and 398.04 ± 13.73 (starter) mg% at day 49. 16S rRNA genes clone libraries were constructed from total DNA extracted from non-starter GJ at day 7, 21, and 42. Nucleotide sequences of Escherichia coli transformants harboring recombinant pGEM-T easy plasmids were analyzed by BLAST. Uncultured bacterium was the most dominant group and Gram – bacteria such as Acidovorax sp., Afipia sp., and Variovorax sp. were the second dominant group. B. amyloliquefaciens (day 7), B. velezensis (day 21 and 42), and B. subtilis (day 42) were observed, but no lactic acid bacteria were detected. Acidovorax and Variovorax species might play some roles for GJ fermentation. Future studies are necessary.

Keywords: Gul jeotgal, Tetragenococcus halophilus, Bacillus subtilis, 16S rRNA clone library

References

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  1. Koo OK, Lee SJ, Chung KR, Jang DJ, Yang HJ, Kwon DY. 2016. Korean traditional fermented fish products: jeotgal. J. Ethn. Foods 3: 107-116.
    CrossRef
  2. Kim CY, Pyeun JH, Nam TJ. 1988. Decomposition of glycogen and protein in pickled oyster during fermentation with salt. Bull. Korean Fish. Soc. 14: 66-71.
  3. Sung NJ. 1978. Degradation of nucleotides and their related compounds during the fermentation of oyster. J. Korean Soc.Food Nutr. 7: 1-6.
  4. Hiau CY, Chai T. 1990. Characterization of oyster shucking liquid wastes and their utilization as oyster soup. J. Food Sci. 55: 374-378.
    CrossRef
  5. Kang KT, Heu MS, Kim JS. 2007. Development of spaghetti sauce with oyster. J. Korean Soc. Food Sci. Nutr. 36: 93-99.
    CrossRef
  6. Kim JA, Yao Z, Kim HJ, Kim JH. 2018. Properties of gul jeotgal (Oyster Jeotgal) prepared with different types of salt and Bacillus subtilis JS2 as starter. Microbiol. Biotechnol. Lett. 46: 1-8.
    CrossRef
  7. Shim JM, Lee KW, Yao Z, Kim JA, Kim HJ, Kim JH. 2017. Microbial communities and physicochemical properties of myeolchi jeotgal (anchovy jeotgal) prepared with different types of salts. J. Microbiol. Biotechnol. 27: 1744-1752.
    Pubmed CrossRef
  8. Yao Z, Liu X, Shim JM, Lee KW, Kim HJ, Kim JH. 2017. Properties of a fibrinolytic enzyme secreted by Bacillus amyloliquefaciens RSB34, isolated from doenjang. J. Microbiol. Biotechnol. 27: 9-18.
    Pubmed CrossRef
  9. Heo OS, Oh SH, Shin HS, Kim MR. 2005. Mineral and heavy metal contents of salt and salted-fermented shrimp. Korean J. Food Sci. Technol. 37: 519-524.
  10. Kim DW, Kim BM, Lee HJ, Jang GJ, Song SH, Lee JI, et al. 2017. Effects of different salt treatments on the fermentation metabolites and bacterial profiles of kimchi. J. Food Sci. 82: 1124-1131.
    Pubmed CrossRef
  11. Gottschalk G. 1986. Bacterial metabolism. pp. 214-224. SpringerVerlag New York Inc.
  12. Graudal N, Jürgens G. 2018. Conflicting evidence on health effects associated with salt reduction calls for a redesign of the salt dietary guidelines. Prog. Cardiovasc. Dis. 61: 20-26.
    Pubmed CrossRef
  13. Kang YM, Chung SK, Paik HD, Cho SH. 2001. Changes in physicochemical component of soy sauce during fermentation from anchovy sauce. J. Korean Soc. Food Sci. Nutr. 30: 888-893.
  14. Hayaloglu AA, Guven M, Fox PF, McSweeney PLH. 2005. Influence of starters on chemical, biochemical, and sensory changes in Turkish white-brined cheese during ripening. J. Dairy Sci. 88: 3460-3474.
    CrossRef
  15. Lee KD, Choi CR, Cho JY, Kim HL, Ham KS. 2008. Physicochemical and sensory properties of salt-fermented shrimp prepared with various salts. J. Korean Soc. Food Sci. Nutr. 37: 53-59.
    CrossRef
  16. Satola B, Wübbeler JH, Steinbüchel A. 2013. Metabolic characteristics of the species Variovorax paradoxus. Appl. Microbiol. Biotechnol. 97: 541-560.
    Pubmed CrossRef
  17. Robertson AW, McCarville NG, Maclntyre LW, Correa H, Haltili B, marchbank DH, et al. 2018. Isolation of imaqobactin, an amphiphilic siderophore from the Arctic marine bacterium Variovorax Species RKJM285. J. Nat. Prod. 81: 858-865.
    Pubmed CrossRef
  18. Han JI, Cho HK, Lee SW, Orwin PM, Kim J, LaRoe SL, et al. 2011. Complete genome sequence of the metabolically versatile plant growth-promoting endophyte Variovorax paradoxus S110. J. Bacteriol. 193: 1183-1190.
    Pubmed KoreaMed CrossRef
  19. Boycheva SS, Pichler FB, Heijstra BD, Lau KEM, Turner SJ. 2015. The genetic toolbox for Acidovorax temperans. J. Microbiol. Methods 115: 129-138.
    Pubmed CrossRef
  20. Cho MS, Jin YJ, Kang BK, Park YK, Kim CK, Park DS. 2018. Understanding the ontogeny and succession of Bacillus velezensis and B. subtilis subsp. subtilis by focusing on kimchi fermentation. Sci. Rep. 8: 7045.
    Pubmed KoreaMed CrossRef
  21. Lee HJ, Chun BH, Jeon HH, Kim YB, Lee SH. 2017. Complete genome sequence of Bacillus velezensis YJ11-1-4, a strain with broad-spectrum antimicrobial activity, isolated from traditional Korean fermented soybean paste. Genome Announc. 5: e01352-17.
    Pubmed KoreaMed CrossRef
  22. Lee JM, Kim YR, Kim JK, Jeong GT, Ha JC, Kong IS. 2015. Characterization of salt-tolerant β-glucosidase with increased thermostability under high salinity conditions from Bacillus sp. SJ-10 isolated from jeotgal, a traditional Korean fermented seafood. Bioproc. Biosyst. Eng. 38: 1335-1346.
    Pubmed CrossRef
  23. Fukui Y, Yoshida M, Shozen KI, Funatsu Y, Takano T, Oikawa H, et al. 2012. Bacterial communities in fish sauce mash using culturedependent and –independent methoods. J. Gen. Appl. Microbiol. 58: 273-281.
    Pubmed CrossRef
  24. Kim MS, Park EJ. 2014. Bacterial communities of traditional salted and fermented seafoods from Jeju island of Korea using 16S rRNA gene clone library analysis. J. Food Sci. 79: M927-M934.
    Pubmed CrossRef
  25. Song EJ, Lee ES, Park SL, Choi HJ, Roh SW, Nam YD. 2018. Bacterial community analysis in three types of the fermented seafood, Jeotgal, produced in south Korea. Biosci. Biotechnol. Biochem. 82:1444-1454.
    Pubmed CrossRef
  26. Maropola MK, Ramond JB, Trindade M. 2015. Impact of metagenomic DNA extraction procedures on the identifiable endophytic bacterial diversity in Sorghum bicolor (L. Moench). J. Microbiol. Methods 112: 104-117.
    Pubmed CrossRef

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