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Microbiology and Biotechnology Letters

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Biocatalysis and Bioprocess Engineering  |  Enzyme, Protein Engineering, and Metabolic Engineering

Microbiol. Biotechnol. Lett. 2019; 47(2): 250-258

https://doi.org/10.4014/mbl.1809.09004

Received: September 14, 2018; Accepted: November 19, 2018

Peniophora sp. JS17 유래 멜라닌 탈색 효소 생산을 위한 배지 조성의 최적화

Optimization of Media Composition on the Production of Melanin Bleaching Enzyme from Peniophora sp. JS17

Min-Jeong Son 1, Yeon-Hee Kim 1, 2, Soo-Wan Nam 1, 2 and Sung-Jong Jeon 1, 2*

1Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering, Dong-Eui University, Republic of Korea, 2Department of Smart-Biohealth, Dong-Eui University, Busan 47340, Republic of Korea

Peniphora sp. JS17, isolated from forest old tree, produced extracellular enzymes that decolorized human hair melanin. The JS17 strain had laccase and manganese peroxidase activity while it did not has lignin peroxidase activity. Batch culture indicated that the melanin decolorization activity of JS17 strain originated from laccase. The culture conditions to maximize the production of melanin bleaching enzymes from Peniophora sp. JS17 mycelia were investigated. Among the tested media for the laccase production, minimal medium (2% glucose, 0.2% malt extract, 0.1% KH2PO4, 0.4% MgSO4·7H2O) showed the highest activity of laccase. Then, to optimize the culture condition for the laccase activity, the influence of various carbon and nitrogen sources was investigated in minimal medium. Among various carbon and nitrogen sources, 2% xylose and 0.4% tryptone showed the highest production of laccase, respectively. The enzyme was purified using (NH4)2SO4 precipitation and Hitrap Q sepharose column, and the purified enzyme showed two isoenzymatic bands with molecular masses of about 70 kDa by SDS-PAGE. The melanin decolorization activity was 77% and 55% within 48 h in the presence of 1-hydroxybenzotriazole (HBT) and syringaldehyde, respectively, whereas only about 9% melanin decolorized in case of no mediator.

Keywords: Melanin, decolorization, Peniophora, laccase, skin whitening

  1. Lin WP, Lai HL, Liu YL, Chiung YM, Shiau CY, Han JM, et al. 2005. Effect of melanin produced by a recombinant Escherichia coli on antibacterial activity of antibiotics. J. Microbiol. Immunol. Infect. 38: 320-326.
  2. Butler MJ, Day AW. 1998a. Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi. Int. J. Plant Sci. 159: 989-995.
    CrossRef
  3. Butler MJ, Day AW. 1998b. Fungal melanins: a review. Can. J. Microbiol. 44: 1115-1136.
    CrossRef
  4. Riley PA. 1997. Melanin. Int. J. Biochem. Cell Biol. 29: 1235-1239.
    CrossRef
  5. Henson JM. 2001. Melanin, p. 229-246. In M. Hofrichter and A. Steinbuechel (ed.), Biopolymers, vol.1, Wiley-VCH Verlag GmbH, Weinheim, Germany.
  6. Al-Saleh I, Shinwari N, El-Doush I, Billedo G, Al-Amodi M, Khogali F. 2004. Comparison of mercury levels in various tissues of albino and pigmented mice treated with two different brands of mercury skin-lightening creams. Biometals 17: 167-175.
    Pubmed CrossRef
  7. Giudice DP, Yves P. 2002. The widespread use of skin lightening creams in Senegal: a persistent public health problem in West. Int. J. Dermatol. 41: 69-72.
    Pubmed CrossRef
  8. Matsubayashi T, Sakaeda T, Kita T, Kurimoto Y, Nakamura T, Nishiguchi K, et al. 2003. Intradermal concentration of hydroquinone after application of hydroquinone ointments is higher than its cytotoxic concentration. Biol. Pharm. Bull. 26:1365-1367.
    Pubmed CrossRef
  9. Rendon MI, Gaviria JI. 2005. Review of skin lightening agents. Dermatol. Surg. 31: 886-889.
    CrossRef
  10. Kaneko S, Cheng M, Murai H, Takenaka S. 2009. Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin. Biosci. Biotechnol. Biochem. 73: 939-942.
    Pubmed CrossRef
  11. Woo SH, Cho JS, Lee BS, Kim EK. 2004. Decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium. Biotechnol. Bioprocess. Eng. 9: 256-260.
    CrossRef
  12. Khammuang S, Sarnthima R. 2013. Decolorization of synthetic melanins by crude laccases of Lentinus polychrous Lév. Folia Microbiol (Praha). 58: 1-7.
    Pubmed CrossRef
  13. Mohorčič M, Friedrich J, Renimel I, Andre P, Mandin D, Chaumont JP. 2007. Production of melanin bleaching enzyme of fungal origin and its application in cosmetics. Biotechnol. Bioprocess Eng. 12: 200-206.
    CrossRef
  14. Nagasaki K, Kumazawa M, Murakami S, Takenaka S, Koike K, Aoki K. 2008. Purification, characterization, and gene cloning of Ceriporiopsis sp. strain MD-1 peroxidases that decolorize human hair melanin. Appl. Environ. Microbiol. 74: 5106-5112.
    Pubmed KoreaMed CrossRef
  15. Kim BS, Blaghen M, Hong HS, Lee KM. 2013. Purification and characterization of a melanin biodegradation enzyme from Geotrichum sp. Int. J. Cosmet. Sci. 38: 622-626.
    Pubmed CrossRef
  16. Cho JS. 2002. Studied on decoloration of melanin using laccase. Available from http://dspace.inha.ac.kr/handle/10505/11037.
  17. Hervé M, Hirschinger J, Granger P, Gilard P. 1994. A 13C solidstate NMR study of the structure and auto-oxidation process of natural and synthetic melanins. Biochim. Biophys. Acta. 1204:19-27.
    CrossRef
  18. Krol ES, Liebler DC. 1998. Photoprotective actions of natural and synthetic melanins. Chem. Res. Toxicol. 11: 1434-1440.
    Pubmed CrossRef
  19. Coconi-Linares N, Magaña-Ortíz D, Guzmán-Ortiz DA, Fernández F, Loske AM, Gómez-Lim MA. 2014. High-yield production of manganese peroxidase, lignin peroxidase, and versatile peroxidase in Phanerochaete chrysosporium. Appl. Microbiol. Biotechnol. 98: 9283-9294.
    Pubmed CrossRef
  20. Archibald FS. 1992. Lignin peroxidase activity is not important in biological bleaching and delignification of unbleached kraft pulp by trametes versicolor. Appl. Environ. Microbiol. 58: 3101-3109.
  21. Baldrian P. 2006. Fungal laccases - occurrence and properties. FEMS Microbiol. Rev. 30: 215-242.
    Pubmed CrossRef
  22. Kenkebashvili N, Elisashvili V, Wasser SP. 2012. Effect of carbon, nitrogen sources, and copper concentration on the ligninolytic enzyme production by Coriolopsis gallica. J. Wast. Conver. Biop. Biotech. 1: 22-27.
  23. Ravikumar G, Gomathi D, Kalaiselvi M, Uma C. 2012. Production, purification and partial characterization of laccase from the mushroom Hypsizygus ulmarius. Int. J. Pharm. Bio. Sci. 3:355-365.
  24. Songulashvili GG, Elisashvili V, Wasser SP, Hadar Y, Nevo E. 2008. Effect of the carbon source and inoculum preparation method on Laccase and manganese peroxidase production in submerged cultivation by the medicinal mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst (Aphyllophoromycetideae). Int. J. Med. Mushrooms 10: 79-86.
    CrossRef
  25. Lee Y, Park CH, Lee BH, Han EJ, Kim TH, Lee JW, et al. 2006. Effect of nutrients on the production of extracellular enzymes for decolorization of reactive blue 19 and reactive black 5. J. Microbiol. Biotechnol. 16: 226-231.
  26. Mikiashvili N, Elisashvili V, Wasser S, Neve E. 2005. Carbon and nitrogen sources influence the ligninolytic enzyme activity of Trametes versicolor. Biotechnol. Lett. 27: 955-959.
    Pubmed CrossRef
  27. Park EH, Yoon KH. 2003. Characterization of laccase purified from Korean Pycnoporus cinnabarinus SCH-3. J. Microbiol. 31:59-66.
    CrossRef
  28. Wang JW, Wu JH, Huang WY, Tan RX. 2005. Laccase production by Monotospora sp., an endophytic fungus in Cynodon dactylon. Bioresour. Technol. 97: 786-789.
    Pubmed CrossRef
  29. Vasconcelos AFD, Barbosa AM, Dekker RFH, Scarminio IS, Rezende MI. 2000. Optimizaton of laccase production by Botryosphaeria sp. in the presence of veratryl alcohol by the response-surface method. Process Biochem. 35: 1131-1138.
    CrossRef
  30. Dhakar K, Pandey A. 2013. Laccase production from a temperature and pH tolerant fungal strain of Trametes hirsuta (MTCC 11397). Enzyme Res. 2013: 869062.
    Pubmed KoreaMed CrossRef
  31. Bettin F, Montanari Q, Calloni R, Gaio TA, Silveira MM, Dillon AJ. 2009. Production of laccases in submerged process by Pleurotus sajor-caju PS-2001 in relation to carbon and organic nitrogen sources, antifoams and Tween 80. J. Ind. Microbiol. Biotechnol. 36:1-9.
    Pubmed CrossRef
  32. Moreira S, Milagres AMF, Mussatto SI. 2014. Reactive dyes and textile effluent decolorization by a mediator system of salt-tolerant laccase from Peniophora cinerea. Sep. Purif. Technol. 135:183-189.
    CrossRef

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