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Microbiology and Biotechnology Letters

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Bioactive Compounds / Food Microbiology  |  Bioactive compounds and Physiological properties

Microbiol. Biotechnol. Lett. 2019; 47(2): 195-200

https://doi.org/10.4014/mbl.1810.10014

Received: October 18, 2018; Accepted: November 11, 2018

물여뀌 에탄올 추출물의 미백 효과

Whitening Activities of Ethanol Extract from Polygonum amphibium L.

Buyng Su Hwang , Seung Young Lee , Chang Hee Kang , Woog Han , Young Taek Oh , Sang Mi Yu , Min Jin Kim , Chul Hwan Kim , Jung Hye Eom , Sang Chul Jeong , Wook Jae Lee , Young Hee Ahn and Yong Tae Jeong *

Nakdonggang National Institute of Biological Resources, Republic of Korea

The purpose of this study was to investigate the melanogenesis inhibiting activity of the ethanol extract from Polygonum amphibium L. Firstly, the n-hexane (Hx), chloroform (CHCl3), ethyl acetate (EA), n-butanol (BuOH), and water (Water) fractions were isolated from the P. amphibium L. ethanol extract. The efficacy of melanogenesis was found to significantly decrease via the EA and BuOH fractions when compared to the control in B16F10 cells. EA particularly showed the lowest melanin content in B16F10 cells when compared to all the other extracts. Concentration-dependent inhibition of melanin synthesis was also observed in the EA fraction at concentrations below 50 μg/ml, which did not exhibit cytotoxicity in B16F10 cells. Notably, the expression of three key proteins (tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2), which are involved in melanogenesis, were significantly decreased via the EA fraction. EA also inhibited body pigmentation in vivo in a zebrafish model. Overall, we demonstrated melanogenesis suppression using the EA fraction from P. amphibium L., which could be a potential candidate for an antimelanogenesis agent.

Keywords: Polygonum amphibium L., melanogenesis, B16F10, zebrafish, ethanol extract

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