Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Biocatalysis and Bioprocess Engineering  |  Enzyme, Protein Engineering, and Metabolic Engineering

Microbiol. Biotechnol. Lett. 2019; 47(1): 78-86

https://doi.org/10.4014/mbl.18012.12015

Received: December 25, 2018; Accepted: December 29, 2018

N-아세틸글루코사민 생산을 위한 코리네박테리움 글루타미컴의

Metabolic Engineering of Corynebacterium glutamicum for N-acetylglucosamine Production

Jin-yeon Kim 1, Bu-yeon Kim 2, Kyung-Ho Moon 1 and Jin-ho Lee 2*

1Department of Pharmacy, Kyungsung University, 2Major in Food Biotechnology, School of Food Biotechnology & Nutrition

Recombinant Corynebacterium glutamicum producing N-acetylglucosamine (GlcNAc) was constructed by metabolic engineering. To construct a basal strain producing GlcNAc, the genes nagA, nagB, and nanE encoding N-acetylglucosamine-6-phosphate deacetylase, glucosamine-6-phosphate deaminase, and Nacetylmannosamine- 6-phosphate epimerase, respectively, were sequentially deleted from C. glutamicum ATCC 13032, yielding strain KG208. In addition, the genes glmS and gna1 encoding glucosamine-6-phosphate synthase and glucosamine-6-phosphate N-acetyltransferase, which originated from C. glutamicum and Saccharomyces cerevisiae, respectively, were expressed in several expression vectors. Among several combinations of glmS and gna1 expression, recombinant cells expressing glmS and gna1 under control of the ilvC promoter produced 1.77 g/l of GlcNAc and 0.63 g/l of glucosamine in flask cultures.

Keywords: N-acetylglucosamine, glucosamine, Corynebacterium glutamicum, metabolic engineering

  1. Hsieh JW, Wu HS, Wei YH, Wang SS. 2007. Determination and kinetics of producing glucosamine using fungi. Biotechnol. Prog. 23: 1009-1016.
    CrossRef
  2. Sitanggang AB, Sophia L, Wu HS. 2012. Aspects of glucosamine production using microorganisms. Int. Food Res. J. 19:393-404.
  3. Liu L, Liu Y, Shin HD, Chen RR, Li J, Du G, et al. 2013. Microbial production of glucosamine and N-acetylglucosamine: advances and perspectives. Appl. Microbiol. Biotechnol. 97: 6149-6158.
    Pubmed CrossRef
  4. Nakamura M, Hikida M, Nakano T, Ito S, Hamano T, Kinoshita S. 1993. Characterization of water retentive properties of hyaluronan. Cornea 12: 433-436.
    Pubmed CrossRef
  5. Park C, Chung KH, Jeong TR, Yang HP, Nam KS, Kim CH. 2000. Effects of N-acetylglucosamine on suppression of collagenolysis and bone resorption in mouse calvarial osteoblasts. J. Chitin Chitosan 5: 79-87.
  6. Cohen-Kupiec R, Chet I. 1998. The Molecular biology of chitin digestion. Curr. Opin. Biotechnol. 9: 270-277.
    CrossRef
  7. Suresh PV. 2012. Biodegradation of shrimp processing biowaste and concomitant production of chitinase enzyme and N-acetyl-D-glucosamine by marine bacteria: production and process optimization. World J. Microbiol. Biotechnol. 28: 2945-2962.
    Pubmed CrossRef
  8. Donzelli BGG, Ostroff G, Harman GE. 2003. Enhanced enzymatic hydrolysis of langostino shell chitin with mixtures of enzymes from bacterial and fungal sources. Carbohydr. Res. 338: 1823-1833.
    CrossRef
  9. Nampoothiri KM, Sandhya TVBC, Sabu A, Szakacs G, Pandey A. 2004. Process optimization for antifungal chitinase production by Trichoderma harzianum. Process Biochem. 39: 1583-1590.
    CrossRef
  10. Sitanggang AB, Wu HS, Wang SS, Ho YC. 2010. Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742. Bioresour. Technol. 101: 3595-3601.
    Pubmed CrossRef
  11. Zhang JX, Liu L, Li JH, Du GC, Chen J. 2012. Enhanced glucosamine production by Aspergillus sp. BCRC 31742 based on the time variant kinetics analysis of dissolved oxygen level. Bioresour. Technol. 111: 507-511.
    Pubmed CrossRef
  12. Deng MD, Severson DK, Grund AD, Wassink SL, Burlingame RP, Berry A, et al. 2005. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine. Metab. Eng. 7: 201-214.
    Pubmed CrossRef
  13. Liu Y, Liu L, Shin HD, Chen RR, Li J, Du G, et al. 2013. Pathway engineering of Bacillus subtilis for microbial production of Nacetylglucosamine. Metab. Eng. 19: 107-115.
    Pubmed CrossRef
  14. Liu Y, Zhu Y, Li J, Shin HD, Chen RR, Du G, et al. 2014. Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production. Metab. Eng. 23: 42-52.
    Pubmed CrossRef
  15. Becker J, Wittmann C. 2012. Bio-based production of chemicals, materials and fuels - Corynebacterium glutamicum as versatile cell factory. Curr. Opin. Biotechnol. 23: 631-640.
    Pubmed CrossRef
  16. Lee J. 2014. Development and characterization of expression vectors for Corynebacterium glutamicum. J. Microbiol. Biotechnol. 24: 70-79.
    Pubmed CrossRef
  17. Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19:selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73.
    CrossRef
  18. van der Rest ME, Lange C, Molenaar D. 1999. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 52: 541-545.
    Pubmed CrossRef
  19. Syukur PH, Kang MS, Ferrer L, Han SS, Lee JY, Kim HS, et al. 2018. Rational engineering of the shikimate and related pathways in Corynebacterium glutamicum for 4-hydroxybenzoate production. J. Biotechnol. 282: 92-100.
    Pubmed CrossRef
  20. Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, et al. 2014. Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetylglucosamine. Appl. Microbiol. Biotechnol. 98: 5633-5643.
    Pubmed CrossRef
  21. Uhde A, Brühl N, Goldbeck O, Matano C, Gurow O, Rückert C, et al. 2016. Transcription of sialic acid catabolism genes in Corynebacterium glutamicum is subject to catabolite repression and control by the transcriptional repressor NanR. J. Bacteriol. 198: 2204-2218.
    Pubmed KoreaMed CrossRef
  22. Mio T, Yamada-Okabe T, Arisawa M, Yamada-Okabe H. 1999. Saccharomyces cerevisiae GNA1, an essential gene encoding a novel acetyltransferase involved in UDP-N-acetylglucosamine synthesis. J. Biol. Chem. 274: 424-429.
    Pubmed CrossRef
  23. Deng MD, Grund AD, Wassink SL, Peng SS, Nielsen KL, Huckins BD, et al. 2006. Directed evolution and characterization of Escherichia coli glucosamine synthase. Biochimie 88: 419-42
    Pubmed CrossRef

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.