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Microbiology and Biotechnology Letters

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Molecular and Cellular Microbiology / Biomedical Sciences  |  Clinical Microbiology and Biomedical Sciences

Microbiol. Biotechnol. Lett. 2019; 47(1): 158-163

https://doi.org/10.4014/mbl.1806.06006

Received: June 22, 2018; Accepted: September 27, 2018

A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus

Tannaz Khodabakhsh 1, Azin Arabi 1, Parviz Pakzad 1, Shivasadat Gheflat 2, Akram Bahreinipour 3 and Mojgan Bandehpour 2, 4*

1Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University, Tehran, Iran, 2Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, 3Iran Veterinary Organization(IVO), Tehran, Iran, 4Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

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Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.

Keywords: Brucella abortus, protein expression, antigenic epitopes, VirB2.3.9, ELISA

References

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