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Microbiology and Biotechnology Letters

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Environmental Microbiology / Microbial Diversity  |  Microbial Ecology and Taxonomy

Microbiol. Biotechnol. Lett. 2019; 47(1): 105-115

https://doi.org/10.4014/mbl.1807.07018

Received: July 24, 2018; Accepted: September 20, 2018

Isolation and Identification of Alkali-tolerant Bacteria from Near-Shore Soils in Dokdo Island

Teddy Namirimu 1, Jinnam Kim 1, 2 and Young-Gun Zo 1, 2*

1Department of Biology, Kyungsung University, Busan 48434 , 2Environmental Science & Technology Research Institute, Kyungsung University, Busan 48434

Abstract

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Saline or alkaline condition in soil inhibits growth of most crop plants and limits crop yields in many parts of the world. Augmenting an alkaline soil with alkali-tolerant bacteria capable of promoting plant growth can be a promising approach in expanding fertile agricultural land. Near-shore environments of Dokdo Island, a remote island located in the middle of the East Sea, appear to have patches of seawater-influenced haloalkaline soil that is unsupportive for growth of conventional plants. To exploit metabolic capacities of alkali-tolerant bacteria for promoting plant growth in saline or alkaline soils, we isolated of alkali-tolerant bacteria from near-shore soil samples in Dokdo and investigated properties of the isolates. Alkali-tolerant bacteria were selectively cultivated by inoculating suspended and diluted soil samples on a plate medium adjusted to pH 10. Fifty colonies were identified based on their GTG5-PCR genomic fingerprints and 16S rRNA gene sequences. Most isolates were affiliated to alkali-tolerant and/or halotolerant genera or species of the phyla Firmicutes (68%), Proteobacteria (30%) and Actinobacteria (2%). Unlike the typical soil bacterial flora in the island, alkali-tolerant isolates belonged to only certain taxa of terrestrial origin under the three phyla, which have traits of plant growth promoting activities including detoxification, phytohormone production, disease/pest control, nitrogen-fixation, phosphate solubilization or siderophore production. However, Firmicutes of marine origin generally dominated the alkali-tolerant community. Results of this study suggest that haloalkaline environments like Dokdo shore soils are important sources for plant growth promoting bacteria that can be employed in bio-augmentation of vegetation-poor alkaline soils.

Keywords: Dokdo, haloalkaline soil, alkali-tolerant bacteria, plant growth promoting bacteria

References

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