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Microbiology and Biotechnology Letters

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Biocatalysis and Bioprocess Engineering  |  Enzyme, Protein Engineering, and Metabolic Engineering

Microbiol. Biotechnol. Lett. 2018; 46(2): 114-119

https://doi.org/10.4014/mbl.1801.01007

Received: January 11, 2018; Accepted: March 16, 2018

Enhanced Production of Astaxanthin by Metabolically Engineered Non-mevalonate Pathway in Escherichia coli

Tae Hyug Jeong 1, Youn Su Cho 2, Seong-Seok Choi 3, Kun-Do Kim 3 and Han Kyu Lim 1*

1Department of Marine and Fisheries Resources, Mokpo National University, 2Department of Fishery biology, Pukyong National University, 3Department of Microbiology, College of Natural Sciences, Pukyong National University

Astaxanthin is one of the major carotenoids used in pigment has a great economical value in pharmaceutical markets, feeding, nutraceutical and food industries. This study was to increase the production of astaxanthin by co-expression with transformed Escherichia coli using six genes involved in the non-mevalonate pathway. Involved in the non-mevalonate biosynthetic pathway of the strain Kocuria gwangalliensis were cloned dxs, ispC, ispD, ispE, ispF, ispG, ispH and idi genes in order to increase astaxanthin production from the transformed E. coli. And co-expression with the genes to compared the amount of astaxanthin production. This engineered E. coli, containing both the non-mevalonate pathway gene and the astaxanthin biosynthesis gene cluster, produced astaxanthin at 1,100 μg/g DCW (dry cell weight), resulting in approximately three times the production of astaxanthin.

Keywords: Astaxanthin, isoprenoid biosynthesis gene, production, Paracoccus haeundaensis, Kocuria gwangalliensis, Escherichia coli

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