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Microbiology and Biotechnology Letters

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Environmental Microbiology / Microbial Diversity  |  Environmental Microbiology

Microbiol. Biotechnol. Lett. 2017; 45(1): 63-70

https://doi.org/10.4014/mbl.1609.09007

Received: September 20, 2016; Accepted: November 4, 2016

독도 심해토 메타게놈 유래 신규 내열성 에스테라아제의 생화학적 특성규명

Biochemical Characterization of a Novel Thermostable Esterase from the Metagenome of Dokdo Islets Marine Sediment

Chang-Muk Lee 1*, Sohyeon Seo 2, Su-Yeon Kim 1, Jaeeun Song 1, Joon-Soo Sim 1, Bum-Soo Hahn 1, Dong-Hern Kim 1 and Sang-Hong Yoon 1

1Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of, 1Global R&D Center, ISU ABXIS, Seongnam 13488, Republic of Korea

Abstract

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A functional screen of 60,672 fosmid metagenomic clones amplified from marine sediment obtained from the Dokdo islets in Korea identified the gene EstES1, whose product, EstES1, displayed lipolytic properties on tributyrin-supplemented media. EstES1 is a 576 amino acid protein with a predicted molecular weight of 59.4 kDa including 37 N-terminal leader amino acids. EstES1 exhibited the highest sequence similarity (44%) to a carboxylesterase found in Haliangium ochraceum DSM14365. Phylogenetic analysis indicated that EstES1 belongs to a currently uncharacterized family of lipases. Within the conserved domain, EstES1 retains the catalytic triad that consists of the consensus penta-peptide motif, GESAG. EstES1 demonstrated a broad substrate specificity toward the long acyl group of ethyl esters (C2−C12), and its optimal activity was recorded toward p-Nitrophenyl butyrate (C4) at pH 9.0 and 40℃ (specific activity of 255.4 U/mg). The enzyme remained stable in the ranges of 60−65℃ and pH 9.0-10.5 and in the presence of methanol, ethanol, isopropanol, and dimethyl sulfoxide. Therefore, EstES1 has potential for use in industrial applications involving high temperature, organic solvents, and/or alkaline conditions.

Keywords: Esterase, thermostable, dokdo island, marine sediment, metagenome

References

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