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Microbiology and Biotechnology Letters

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Biocatalysis and Bioprocess Engineering  |  Bioenergy and Biorefinery

Microbiol. Biotechnol. Lett. 2017; 45(1): 51-56

https://doi.org/10.4014/mbl.1702.02005

Received: February 23, 2017; Accepted: March 21, 2017

유도적 돌연변이 유발 방법을 통한 1-ethyl-3-methylimidazolium acetate에 대해 내성을 갖는 돌연변이 효모 선별

Isolation of Mutant Yeast Strains having Resistance to 1-ethyl-3-methylimidazolium Acetate through a Directed Evolutionary Approach

Yoo-Jin Lee 1, Deok-Ho Kwon 1, Jae-Bum Park 1 and Suk Jin Ha 1*

Department of Bioengineering and Technology, Kangwon National University, Chuncheon 24341, Republic of Korea

Cellulosic biomass is a renewable source for biofuel production from non-edible biomass. An optimized pretreatment process is required for the efficient utilization of cellulosic biomass. Among various pretreatment processes, the use of ionic liquids has been reported recently. However, the residual ionic liquid after pretreatment acts as an inhibitor of microbial fermentation. Recently, we isolated mutant Saccharomyces cerevisiae strains resistant to the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]) by using a directed evolutionary approach. When 3% [EMIM][Ac] was added to a medium containing 80 g/l of glucose, mutants D452-B2 and D452-S3 produced 35.6 g/l and 36.3 g/l of ethanol, respectively, for 18 h while the parental strain (S. cerevisiae D452-2) produced 1.3 g/l of ethanol. Thus, these mutant S. cerevisiae strains might prove advantageous when ionic liquids are used for biofuel production from cellulosic biomass.

Keywords: cellulosic biomass, ionic liquid, tolerance, yeast

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