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Microbiology and Biotechnology Letters

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Bioactive Compounds / Food Microbiology  |  Probiotics and Foodborne Microorganisms

Microbiol. Biotechnol. Lett. 2017; 45(1): 35-42

https://doi.org/10.4014/mbl.1701.01002

Received: February 1, 2017; Accepted: March 24, 2017

프로바이오틱 유산균 발효에 의한 배초향 잎 추출물의 피부 항산화 및 항염증 활성 증대

Enhancement of Skin Antioxidant and Anti-Inflammatory Potentials of Agastache rugosa Leaf Extract by Probiotic Bacterial Fermentation in Human Epidermal Keratinocytes

Hye-Won Lim 1, Yoonjin Lee 2, Yu-Hua Huang 1, Ji-Young Yoon 3, Su Hee Lee 4, Kyunghoon Kim 4 and Chang-Jin Lim 1, 5*

1Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea, 2R&D Center, Cosmocos Corporation, Incheon 21698, Republic of Korea, 3Jeonju AgroBio-Materials Institute, Joenju 54810, Republic of Korea, 4Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea, 5Department of Biochemistry, Kangwon National University, Chuncheon 24341, Republic of Korea

This study aimed to investigate the effects of probiotic fermentation by comparing the skin antioxidant and anti-inflammatory properties of non-fermented (ARE) and fermented (ARE-F) hot water extracts of Agastache rugosa leaves. ARE-F was obtained via ARE fermentation using Lactobacillus rhamnosus HK-9. In vitro, anti-inflammatory properties were evaluated by analyzing the levels of nitric oxide (NO), reactive oxygen species (ROS), and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated HaCaT keratinocytes. In vitro antiradical activity was measured using 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Attenuation of LPS-stimulated NO (p < 0.01), ROS (p < 0.001) and iNOS (p < 0.05) levels by ARE-F was significantly stronger than that by ARE in HaCaT keratinocytes. However, no differences were observed between the DPPH radical scavenging activities of ARE and ARE-F. ARE-F possesses enhanced skin antioxidant and anti-inflammatory properties, suggesting that probiotic bacterial fermentation can be considered an effective tool for augmenting some pharmacological properties of A. rugosa leaves. In brief, the skin antioxidant and anti-inflammatory potentials of A. rugosa leaf extract are augmented by the fermentation with L. rhamnosus HK-9, a probiotic bacterium.

Keywords: Agastache rugosa, inducible nitric oxide synthase, nitric oxide, reactive oxygen species, anti-inflammatoy, antiradical

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