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Microbiology and Biotechnology Letters

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Environmental Microbiology / Microbial Diversity  |  Environmental Microbiology

Microbiol. Biotechnol. Lett. 2016; 44(4): 540-549

https://doi.org/10.4014/mbl.1608.08004

Received: August 4, 2016; Accepted: October 12, 2016

토양 식물 잔사에서 Trichoderma harzianum에 의한 식물 병원균 Fusarium solani의 성장 저해

Growth Competition between Trichoderma harzianum and Fusarium solani on a Plant Residue in Non-Sterile Soil

Tae Gwan Kim 1* and Guy Knudsen 2

1Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 46241, Republic of Korea, 2Soil and Land Resources Division, Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow, ID 83844-2339, USA

Abstract

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Plant residues serve as substrates for the proliferation and overwintering of plant pathogenic fungi in soil. Effects of the biocontrol fungus Trichoderma harzianum on the colonization of wheat straw by the plant pathogenic fungus Fusarium solani were investigated under different soil moisture regimes (−50 vs. −500 kPa) in non-sterile soil. T. harzianum ThzID1-M3 and/or F. solani were added along with wheat straw to non-sterile soils. ThzID1-M3, other Trichoderma species, and F. solani were monitored for a 21-day period using quantitative PCR. ThzID1-M3 reduced the colonization of F. solani on wheat straw (p < 0.05) under both moisture regimes, and F. solani reduced the colonization by ThzID1-M3 and other Trichoderma species (p < 0.05), thus suggesting competitive inhibition between ThzID1-M3 and F. solani. Colonization by ThzID1-M3 and generic Trichoderma was improved in the wet soil (p < 0.05), but colonization by F. solani did not differ between the two moisture conditions. Thus, the inhibitory effect of ThzID1-M3 was greater in the wet soil (p < 0.05). The growth competition between ThzID1-M3 and F. solani to colonize plant debris suggests that the biocontrol fungus T. harzianum may reduce the potential of the plant pathogen, F. solani, to survive and proliferate on crops.

Keywords: Trichoderma harzianum, Fusarium solani, competition, colonization, wheat straw, biological control

References

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