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Microbiology and Biotechnology Letters

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Bioactive Compounds / Food Microbiology  |  Food Biotechnology

Microbiol. Biotechnol. Lett. 2016; 44(4): 452-460

https://doi.org/10.4014/mbl.1609.09002

Received: September 8, 2016; Accepted: October 25, 2016

함초 씨의 항균, 항산화 및 항혈전 활성

Antimicrobial, Antioxidant, and Anticoagulation Activities of Salicornia europaea seeds

Ho-Yong Sohn 1*, Mi-Sun Kim 1 and Deuk Hoi Kim 2

1Department of Food and Nutrition, Andong National University, Andong 36729, Republic of Korea, 2Research Center, Phyto Corporation, Seoul 08826, Republic of Korea

Abstract

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This study was designed to develop a functional pharma-food using Salicornia europaea (SE). Tiny seeds from the mature SE were collected, and their biological activities were evaluated. The extraction yield of the seed in hot water was found to be 29.6% and the hot water extract (HWE) contained 25.7 mg/g total polyphenol (TP) and 11.5 mg/g total flavonoid (TF), which are similar to those contained in leaf and stem of SE. Among the subsequent organic solvent fractions, the ethylacetate (EA) fraction exhibited the highest content of TP (158.3 mg/g), TF (136.2 mg/g), and total sugar (228.3 mg/g). The EA fraction exhibited broad-range antibacterial activities against gram-positive bacteria, and the butanol fraction exhibited growth inhibitory effect against only Staphylococcus epidermidis. An antioxidation activity assay of the HWE and its fractions showed the EA fraction to have the highest radical scavenging activity with RC50 values of 57.0, 29.0, and 28.9 μg/ml against DPPH anion, ABTS cation, and nitrite, respectively. The RC50 values of vitamin C against DPPH anion, ABTS cation, and nitrite were 10.7, 4.0, and 18.0 μg/ml, respectively, indicating that the EA fraction of SE has potent antioxidant compounds. In an anticoagulation assay, the EA fraction exhibited a 15-fold extended thrombin time at 5 mg/ml and activated partial thromboplastin time at 7 mg/ ml, which are comparable to the activities of aspirin. The HWE and its fractions had no hemolysis activities against human RBCs at up to 1 mg/ml. These results suggest that the EA fraction from SE has a great potential as a new antibacterial and anticoagulation agent.

Keywords: anti-microbial, anti-oxidation, anti-thrombosis, seed, Salicornia europaea

References

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