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Microbiology and Biotechnology Letters


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Enzymes and Bioactive Compounds

Microbiol. Biotechnol. Lett. 2015; 43(4): 336-342

Received: September 1, 2015; Accepted: December 1, 2015

Euptelea pleiosperma 에탄올 추출물의 항비만 활성

Anti-Obesity Activity of Euptelea Pleiosperma Ethanol Extract

Jung Ae Park 1, Kyong-Suk Jin 1, Hyun Ju Kwon 1, 2 and Byung Woo Kim 1, 2*

1Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan 47340, Republic of Korea, 2Department of Life Science and Biotechnology, College of Natural Science and Human Ecology, Dong-Eui University, Busan 47340, Republic of Korea

Previously, Euptelea pleiosperma was identified as one of the useful sources containing anti-oxidative and anti-inflammatory activities for the first time in our research group. In this study, anti-obesity effect of E. pleiosperma ethanol extract (EPEE) was evaluated by using a pancreatic lipase enzyme inhibition assay and a cell culture model system. EPEE suppressed effectively pancreatic lipase enzyme activity dose dependently. Furthermore, EPEE significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride contents, and triggered lipolysis activity on 3T3-L1 preadipocytes in a dose-dependent manner without cytotoxicity. Anti-adipogenic effect of EPEE was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/ enhancer binding proteins α (C/EBPα), C/EBPβ and peroxisome proliferator-activated receptor γ (PPARγ) gene and protein expressions. Taken together, these results provide the important new insight that E. pleiosperma possesses anti-obesity activities such as pancreatic lipase inhibition, anti-adipogenic, and lipolysis effects. It might be utilized as promising sources in the fields of nutraceuticals. The identification of active compounds that confer anti-obesity activity of EPEE might be needed.

Keywords: Euptelea pleiosperma, anti-obesity activity, lipase inhibition activity, anti-adipogenic and lipolysis effects

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