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Microbiology and Biotechnology Letters

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Enzymes and Bioactive Compounds

Microbiol. Biotechnol. Lett. 2014; 42(3): 293-296

https://doi.org/10.4014/kjmb.1407.07004

Received: July 10, 2014; Accepted: August 6, 2014

극지 미생물들의 배양온도에 따른 성장률 및 protease activity 영향 연구

Effect of Temperature on Growth Rate and Protease Activity of Antarctic Microorganisms

Hyun-do Kim 1 and Jong-il Choi 1*

Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 500-757, Republic of Korea

This study was conducted to investigate the effect of culture temperature on the growth rate and protease activity of Antarctic microorganisms. The Antarctic microorganisms PAMC 25641, 25614, 25719 and 25617 were obtained from the Polar and Alpine Microbial Collection (PAMC) at the Korea Polar Research Institute. These microorganisms were confirmed for the excretion of protease on a plate with skim milk. The identification of microorganisms was carried out using the 16S rDNA sequencing method. PAMC 25641 showed the highest protease activity among the subjects tested, and PAMC 25617 exhibited the highest growth rate. The growth rates of the microorganisms were not affected by temperature, except for PAMC 25617. However, protease activities were increased for all strains in a temperature dependent fashion. These results suggest the possible application of Antarctic microorganisms for the efficient production of low temperature proteases.

Keywords: Protease, growth rate, Antarctic microorganisms, culture temperature

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