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Microbiology and Biotechnology Letters

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Molecular and Cell biology, and Microbial Isolation

Microbiol. Biotechnol. Lett. 2014; 42(3): 211-218

https://doi.org/10.4014/kjmb.1406.06001

Received: June 9, 2014; Accepted: August 6, 2014

장내 유해세균을 억제하는 양돈용 프로바이오틱스 개발을 위한 비피도박테리아 탐색

Screening of Bifidobacteria for the Development of Probiotics Inhibiting Intestinal Pathogenic Bacteria

Jaeyeon Lee 1, Yungoh Shin 1 and Keun Kim 1*

Institute of Bioindustry, The University of Suwon, Hwaseong 445-743, Republic of Korea

In order to isolate probiotic lactic acid bacteria possessing high inhibitory activities against porcine and zoonotic pathogens, such as enterotoxigenic E. coli, Salmonella Typhimurium, and Clostridium perfringens, a total of 65 anaerobic strains were initially isolated from a variety of sources including cattle rumen fluids, chicken intestines and swine feces. Four Bifidobacterium strains were selected for their high anti-pathogenic bacterial activities. By using the 16S rDNA sequencing method, three B. boum strains and one B. thermophilum were identified. B. thermophilum demonstrated the best adhesive ability to epithelial cells of swine intestine among the isolates. Indeed, B. thermophilum was seen to have superior characteristics as a probiotic for swine, as judged by their high growth inhibitory activities against various pathogens, and high acid- and bile-tolerance.

Keywords: Lactic acid bacteria, probiotics, swine, intestinal pathogenic bacteria

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