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Microbiology and Biotechnology Letters


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Fermentation and Bioprocess Engineering

Microbiol. Biotechnol. Lett. 2014; 42(2): 114-120

Received: April 7, 2014; Accepted: April 28, 2014

주목 식물세포(Taxus chinensis) 배양 유래 Paclitaxel 정제를 위한 분별침전에서 제타전위 영향

Effect of Zeta Potential on Fractional Precipitation for the Purification of Paclitaxel from Plant Cell Cultures of Taxus chinensis

Heung Kon Ryu 1 and Jin-Hyun Kim 1*

Department of Chemical Engineering, Kongju National University, Cheonan 331-717, Republic of Korea

This study evaluated the effect of the zeta potential of silica-alumina on the behavior, in terms of purity, yield, fractional precipitation time, precipitate shape, size of fractional precipitation in the increased surface area, and the fractional precipitation process, for the purification of paclitaxel. As the zeta potential value of silica-alumina increased, the yield of paclitaxel concurrently increased while the precipitation time decreased. The use of alumina with the highest value of the zeta potential (+35.41 mV) as a surface area-increasing material dramatically reduced the precipitation time by 12 h compared with the results of the control. On the other hand, the purity of paclitaxel had almost no effect on changes in the zeta potential of silicaalumina. In addition, the precipitate size was inversely correlated with the absolute value of the zeta potential.

Keywords: Paclitaxel, Purification, Fractional precipitation, Silica-alumina, Zeta potential

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