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Microbiology and Biotechnology Letters


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Fermentation and Bioprocess Engineering

Microbiol. Biotechnol. Lett. 2013; 41(4): 407-415

Received: November 1, 2013; Accepted: December 9, 2013

황칠나무 잎 추출물의 세포 항산화 활성과 미백활성 측정

Cellular Antioxidant Activity and Whitening Effects of Dendropanax morbifera Leaf Extracts

Su Ah Park 1, Jun Park 2, Chan Il Park 2, Young Jong Jie 2, Yun Chan Hwang 2, Yong Hyun Kim 2, So Ha Jeon 1, Hye Mi Lee 1, Ji Hoon Ha 1, Kyeong Jin Kim 1 and Soo Nam Park 1*

1Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology, Seoul 139-743, Korea, 2Seoul Science High School, Seoul 110-521, Korea

In this study, we investigated the antioxidant activities on HaCaT and the whitening effects on B16F1 melanoma cells of Dendropanax morbifera leaf extract. In an antioxidative activity assay using HaCaT cells, the ethyl acetate (50 μg/ml) and aglycone fractions (25 μg/ml) of the D. morbifera leaf extract didn't exhibit any characteristics of cytotoxicity. When HaCaT cells were exposed to a single large dose (800 mJ/cm2) of UVB, the extracts protected the cells against UVB radiation. When HaCaT cells were treated with 10 mM H2O2 and 4 μM rose bengal, the ethyl acetate (6.25~50 μg/ml) and aglycone (6.25~25 μg/ml) fractions protected the cells against oxidative damage in a concentration dependent manner. When the whitening effects of D. morbifera leaf extract were tested in melanoma B16/F1 cells treated with the a-melanocyte stimulating hormone (α-MSH), the extracts inhibited α-MSH-stimulated intra/extracellular melanogenesis in a concentration dependent manner. The inhibitory effects of the ethyl acetate and aglycone fractions of D. morbifera leaf extract were 21% and 44% at 25 μg/ml, respectively. Both are more effective than arbutin (15% at 25 μg/ml) which is known as a whitening agent. These results indicate that fractions of the D. morbifera leaf can function as cell protectants and natural antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging 1O2 and other ROS, and protecting cells against ROS. In addition, fractions of the D. morbifera leaf can be applied to new whitening cosmetics because of their inhibitory effects on α-MSH stimulated melanogenesis in B16F1 melanoma cells.

Keywords: Dendropanax morbifera, antioxidant activity, HaCaT, B16F1 melanoma, a-MSH, melanogenesis

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