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Microbiology and Biotechnology Letters

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Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2013; 41(3): 341-349

https://doi.org/10.4014/kjmb.1306.06002

Received: June 26, 2013; Accepted: August 9, 2013

통초 · 희렴 추출물의 항산화 · 항비만 활성 및 혼합물의 항비만 시너지 효과

Anti-Oxidative and Anti-Obesity Activities of Tetrapanax papyriferus and Siegesbeckia pubescens Extracts and their Synergistic Anti-Obesity Effects

Jung Ae Park 1, Kyong-Suk Jin 1, Ji Young Lee 1, Hyun Ju Kwon 1, 2 and Byung Woo Kim 1, 2*

1Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan, 614-714, Korea, 2Department of Life Science and Biotechnology, College of Natural Science, Dong-Eui University, Busan, 614-714, Korea

Abstract

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In this study, the anti-oxidative and anti-obesity activities of two medicinal herb extracts, Tetrapanax papyriferus (TP) and Siegesbeckia pubescens (SP), were evaluated using DPPH radical scavenging activity assay, lipase enzyme inhibition assay, and the cell culture model system. Both methanol extracts of TP and SP showed DPPH radical scavenging activities dosedependently, and the IC50 of DPPH radical scavenging activities of the two medicinal herbs were 65.23 and 47.79 μg/ml, respectively. Furthermore, both extracts suppressed effectively lipase enzyme activity dose-dependently. Moreover, TP and SP extracts significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents on 3T3-L1 preadipocytes in a dose-dependent manner without cytotoxicity. Their anti-obesity effect was modulated by cytidine-cytidine-adenosineadenosine- thymidine (CCAAT)/enhancer binding proteins α (C/EBPα), C/EBPβ and peroxisome proliferator-activated receptor γ (PPARγ) gene and protein expressions. Furthermore, TP and SP possessed a synergistic effect on anti-obesity activity. The identification of the active compounds that confer the anti-obesity activity of TP and SP might be needed.

Keywords: Tetrapanax papyriferus, Siegesbeckia pubescens, anti-oxidative effect, lipase inhibition activity, anti-obesity activity, synergic effect

References

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