Article Search
닫기

Microbiology and Biotechnology Letters

단보(Note)

View PDF

Note

Microbiol. Biotechnol. Lett. 2013; 41(2): 253-262

https://doi.org/10.4014/kjmb.1207.07022

Received: July 27, 2012; Accepted: April 17, 2013

통초, 필발을 포함한 7종 한약재 추출물의 항산화 및 항염증 활성

Anti-Oxidative and Anti-Inflammatory Activities of Seven Medicinal Herbs including Tetrapanax papyriferus and Piper longum Linne

Kyong-Suk Jin 1, You Na Oh 1, Ji Young Lee 1, Byung Yil Son 1, Woobong Choi 1, 2, Eun-Woo Lee 1, 3, Hyun Ju Kwon 1, 3 and Byung Woo Kim 1, 3*

1Blue-Bio Industry Regional Innovation Center, Dongeui University, Busan, 614-714, Korea, 2Department of Life Science and Biotechnology, College of Natural Science, Deong-Eui University, Busan, 614-714, Korea, 3Department of Life Science and Biotechnology, College of Natural Science, Deong-Eui University, Busan, 614-714, Korea

In this study, we analyzed the anti-oxidative and anti-inflammatory activities of seven medicinal herbs. All extracts of the tested herbs, Euryale ferox Salisbury, Echinops setifer Iljin, Amomum cardamomum Linne, Tetrapanax papyriferus, Illicium verum Hook. f., Typha orientalis Presl, and Piper longum Linne, exhibited potent anti-oxidative activity as confirmed by DPPH radical scavenging capacity. Lipopolysaccharide (LPS) induced nitric oxide (NO) production, in the RAW 264.7 cell line, was also ameliorated by all extracts’ treatments in a dose dependent manner. NO suppressive activity originated from the inhibition of inducible nitric oxide synthase (iNOS) protein expression by the extracts. Three extracts, E. ferox S., I. verum Hook. f., and P. longum L., possessed suppressive activity against, not only iNOS, but also cycloxygenase 2 (COX-2) protein expression. These three extracts may then serve as potential candidates for non steroidal analgesic inflammation drugs (NSAIDs). Furthermore, all extracts induced anti-oxidative enzyme, heme oxygenase 1, protein expression. Taken together, these results provide an important new insight into the fact that various medicinal herbs possess potent anti-oxidative and anti-inflammatory activities and might be utilized as promising agents in the field of health products. Further studies for the identification of the active compounds from medicinal herbs are clearly needed.

Keywords: medicinal herbs, anti-oxidative activity, anti-inflammatory activity, nitric oxide, inducible nitric oxide synthase, cycloxygenase-2

  1. Aktan, F. 2004. iNOS-mediated nitric oxide production and its regulation. Life Sci. 75: 639-653.
    Pubmed CrossRef
  2. Bae, G. S., M. S. Kim, W. S. Jung, S. W. Seo, S. W. Yun, S. G. Kim, R. K. Park, E. C. Kim, and H. J. Song. 2010. Inhibition of lipopolysaccharide-induced inflammatory responses by piperine. Eur. J. Pharmacol. 642: 154-162.
    Pubmed CrossRef
  3. Bhadra, S., P. K. Mukherjee, N. S. Kumar, and A. Bandyopadhyay. 2011. Anticholinesterase activity of standardized extract of ollicium verum Hook. f. fruits. Fitoterapia. 82: 342346.
    Pubmed CrossRef
  4. Brand-Williams, W., M. E. Cuvelier, and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. 28: 25-30.
  5. Chawla, A., K. D. Nguyen, and Y. P. Goh. 2011. Macrophagemediated inflammation in metabolic disease. Nat. Rev. Immunol. 11: 738-749.
    Pubmed KoreaMed CrossRef
  6. Ge, J., D. Wang, R. He, H. Zhu, Y. Wang, and S. He. 2010. Medicinal herb research: serum pharmacological method and plasma pharmacological method. Biol. Pharm. Bull. 33: 14591465.
    CrossRef
  7. Grivennikov, S. I., F. R. Greten, and M. Karin. 2010. Immunity, inflammation, and cancer. Cell 140: 883-899.
    Pubmed KoreaMed CrossRef
  8. Han, Z., J. Luo, and L. Y. Kong. 2012. Two new tocopherol polymers from the seeds of Euryale ferox. J. Asian Nat. Prod. Res. 14: 743-747.
    Pubmed CrossRef
  9. Huang, G. J., J. S. Deng, J. C. Liao, W. C. Hou, S. Y. Wang, P. J. Sung, and Y. H. Kuo. 2012. Inducible nitric oxide synthase and cyclooxygenase-2 participate in anti-inflammatory activity of imperatorin from Glehnia littoralis. J. Agric. Food Chem. 60:1673-1681.
    Pubmed CrossRef
  10. Huang, Y., J. Zhao, L. Zhou, J. Wang, Y. Gong, X. Chen, Z. Guo, Q. Wang, and W. Jiang. 2010. Antifungal activity of the essential oil of Illicium verum fruit and its main component transanethole. Molecules. 15: 7558-7569.
    Pubmed CrossRef
  11. Jang, M. J., M. H. Woo, Y. H. Kim, D. Y. Jun, and W. J. Rhee. 2005. Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of Sancho (Zanthoxylum schinifolium). Korean J. Nutri. 38: 386-394.
  12. Jin, K. S., J. Y. Park, M. K. Cho, J. H. Jang, J. H. Jeong, S. Ok, M. J. Bak, Y. S. Song, M. J. Kim, C. W. Cho, and W. S. Jeong. 2009. Modulation of Nrf2/ARE and inflammatory signaling pathways by Hericium erinaceus mycelia extract. Food Sci. Biotechnol. 18: 1204-1211.
  13. Kim, K. H., I. K. Kang, E. J. Kang, E. K. Yang, and S. N. Park. 2004. A research trend of natural product on well-being industry. J. Soc. Cosmet. Scientists Korea 30: 329-343.
  14. Kim, Y. H., M. J. Lee, H. S. Lee, J. G. Kim, and W. H. Park. 2011. Suppressive effect of Euryale ferox Salisbury extracts on inflammatory response in LPS- stimulated RAW 264.7 cells through the antioxidative mechanism. Korean J. Ori. Physiol. Pathol. 25: 202-211.
  15. Khor, T. O., S. Yu, and A. N. Kong. 2008. Dietary cancer chemopreventive agents - targeting inflammation and Nrf2 signaling pathway. Planta Med. 74: 1540-1547.
    Pubmed CrossRef
  16. Kumari, M., B. K. Ashok, B. Ravishankar, T. N. Pandya, and R. Acharya. 2012. Anti-inflammatory activity of two varieties of Pippali (Piper longum Linn.). Ayu. 33: 307-310.
    Pubmed KoreaMed CrossRef
  17. Kundu, J. K. and Y. J. Surh. 2008. Inflammation: gearing the journey to cancer. Mutat. Res. 659: 15-30.
    Pubmed CrossRef
  18. Kundu, J. K. and Y. J. Surh. 2012. Emerging avenues linking inflammation and cancer. Free Radic. Biol. Med. 52: 20132037.
    Pubmed CrossRef
  19. Lee, B. C., H. M. Park, H. S. Sim, G. S. Kim, J. H. Gu, and M. J. Oh. 2009. Biological activity and chemical analysis of cattail pollens. J. Agri. Sci. 36: 185-197.
  20. Lee, D. H., S. S. Kang, I. M. Chang, and W. Mar. 1997. Detection of anti-inflammatory agents from natural products as inhibitors of cyclooxygenase I and II. Nat. Prod. Sci. 3: 19-28.
  21. Lee, M. S. 2004. Looking at ‘Well-Being’ in terms of lifestyle:Healthy or trendy- Korean Living Science Association 13:477-484.
  22. Lee, S. E., E. M. Ju, and J. H. Kim. 2002. Antioxidant activity of extracts from Euryale ferox seed. Exp. Mol. Med. 34: 100106.
    Pubmed CrossRef
  23. Lipinski, B. 2011. Hydroxy radical and its scavengers in health and disease. Oxid. Med. Cell Longev. 2011: 809696, 9 pages.
  24. Muller, N., A. M. Myint, and M. J. Schwarz. 2011. Inflammatory biomarkers and depression. Neurotox. Res. 19: 308-318.
    Pubmed CrossRef
  25. Nam, S. H. and M. Y. Kang. 2000. Screening of antioxidative activity of hot-water extracts from medicinal plants. J. Korean Soc. Agric. Chem. Biotechnol. 43: 141-147.
  26. Nicoletti, M. 2012. Nutraceuticals and botanicals: overview and perspectives. Int. J. Food Sci. Nutr. 63: 2-6.
    Pubmed CrossRef
  27. Ng, T. B., F. Liu, and Z. T. Wang. 2000. Antioxidative activity of natural products from plants. Life Sci. 14: 709-723.
    CrossRef
  28. Ngamwongsatit, P., P. P. Banada, W. Panbangred, and A. K. Bhunia. 2008. WST-1-based cell cytotoxicity assay as as substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J. Microbiol. Methods 73:211-215.
    Pubmed CrossRef
  29. Park, C. M., J. Y. Park, K. H. Noh, J. H. Shin, and Y. S. Song. 2011. Taraxacum officinale Weber extracts inhibit LPSinduced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells. J. Ethnopharmacol. 133: 834-842.
    Pubmed CrossRef
  30. Park, C. M., K. S. Jin, Y. W. Lee, and Y. S. Song. 2011. Luteolin and chicoric acid synergistically inhibited inflammatory responses via inactivation of PI3K-Akt pathway and impairment of NF-κB translocation in LPS stimulated RAW 264.7 cells. Eur. J. Pharmacol. 660: 454-459.
    Pubmed CrossRef
  31. Perez, G. 2001. Anti-inflammatory activity of compounds isolated from plants. TheScientificWorld 1: 713-784.
    Pubmed CrossRef
  32. Prochazkova, D., I. Bousova, and N. Wilhelmova. 2011. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 82: 513-523.
    Pubmed CrossRef
  33. Roh, E. J., B. K. Kim, and D. S. Kim. 2011. Antioxidative activity and antiaging effects of Tetrapanax papyriferum extract. J. Korean Oil Chemists' Soc. 28: 219-224.
  34. Row, L. C., J. C. Ho, and C. M. Chen. 2007. Cerebrosides and tocopherol trimers from the seeds of Euryale ferox. J. Nat. Prod. 70: 1214-1217.
    Pubmed CrossRef
  35. Ryu, M. J., S. Y. Lee, Y. Park, and Y. K. Yang. 2010. Antioxidative activities and antifungal effect against Malassezia furfur in the extracts from 6 spp. Medicinal plants. J. Korean Soc. Cosm. 16: 120-128.
  36. Song, C. W., S. M. Wang, L. L. Zhou, F. F. Hou, K. J. Wang, Q. B. Han, N. Li, and Y. X. Cheng. 2011. Isolation and identification of compounds responsible for antioxidant capacity of Euryale ferox seeds. J. Agric. Food Chem. 59: 1199-1204.
    Pubmed CrossRef
  37. Sung, Y. Y., W. K. Yang, A. Y. Lee, D. S. Kim, K. J. Nho, Y. S. Kim, and H. K. Kim. 2012. Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice. J. Ethnopharmacol. 144: 151-159.
    Pubmed CrossRef
  38. Sung, Y. Y., Y. S. Kim, and H. K. Kim. 2012. Illicium verum extract inhibits TNF-α-and IFN-γ-induced expression of chemokines and cytokines in human keratinocytes. J. Ethnopharmacol. 144: 182-189.
    Pubmed CrossRef
  39. Surh, Y. J. 2003. Cancer chemoprevention with dietary phytochemicals. Nat. Rev. Cancer 3: 768-780.
    Pubmed CrossRef
  40. Verdon, C. P., B. A. Burton, and R. L. Prior. 1995. Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the griess reaction is used to assay for nitrite. Anal. Biochem. 224: 502-508.
    Pubmed CrossRef
  41. Wachtel-Galor, S. and I. F. F. Benzie. 2011. Herbal Medicine:an introduction to its history, usage, regulation, current trends, and research needs. Herbal Medicine: biomolecular and clinical aspects. 2nd edition. Boca Raton (FL): CRC Press, Chapter 1.
  42. Wang, G. W., W. T. Hu, B. K. Huang, and L. P. Qin. 2011. Illicium verum: a review on its botany, traditional use, chemistry and pharmacology. J. Ethnopharmacol. 136: 10-20.
    Pubmed CrossRef
  43. Yang, J. F., C. H. Yang, H. W. Chang, C. S. Yang, S. M. Wang, M. C. Hsieh, and L. Y. Chuang. 2010. Chemical composition and antibacterial activities of Illicium verum against antibioticresistant pathogens. J. Med. Food. 13: 1254-1262.
    Pubmed CrossRef

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.