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Microbiology and Biotechnology Letters

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Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2013; 41(2): 207-214

https://doi.org/10.4014/kjmb.1301.01001

Received: January 3, 2013; Accepted: February 12, 2013

미세조류 옥외 배양시스템을 이용한 바이오디젤 생산 및 도시하수 영양 염류 제거

Production of Biodiesel and Nutrient Removal of Municipal Wastewater using a Small Scale Raceway Pond

Zion Kang 1, 2, Byung-Hyuk Kim 1, Hee-Mock Oh 1, 2 and Hee-Sik Kim 1, 2*

1Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea, 2University of Science and Technology (UST), Daejeon 303-333, Korea

A concerted effort to develop alternative forms of energy is underway due to fossil fuel shortages and its deleterious effects. Recently, bioenergy from microalgae has gained prominence and the use of municipal wastewater as a low cost alternative for a nutrient source has significant advantages. In this study, we have employed municipal wastewater directly after primary treatment (primary settling basin) in a small scale raceway pond (SSRP) for microalgal growth. Indigenous microalgae in the wastewater were encouraged to grow in the SSRP under optimal conditions. The mean removal efficiencies of TN, TP, and NH3-N after 6 days were 77.77%, 63.55%, and 89.02%, respectively. The average lipid content of the microalgae was 19.51% of dry cell weight, and linolenate and linoleate (18:n) were the predominant fatty acids. The 18S rRNA gene analysis and microscopic observations of the indigenous microalgae community revealed the presence of Chlorella vulgaris and Scenedesmus obliquus as the dominant microalgae. These results indicate that untreated municipal wastewater, serving as an excellent nitrogen and phosphate source for microalgal growth, could be treated using microalgae in open raceway ponds. Moreover, microalgal biomass could be further profitable by the extraction of biodiesel.

Keywords: microalgae, biodiesel, wastewater treatment, small scale raceway pond (SSRP)

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