Article Search

Microbiology and Biotechnology Letters


View PDF

Enzymes and Bioactive Compounds

Microbiol. Biotechnol. Lett. 2013; 41(1): 44-51

Received: October 29, 2012; Accepted: January 3, 2013

Corallococcus와 Myxococcus 속 점액세균 균주들에 의한 항균 물질의 생산

Production of Antimicrobial Substances by Strains of Myxobacteria Corallococcus and Myxococcus

Hyejin Shin 1, Jinkwon Youn 1, Dongju An 1 and Kyungyun Cho 1*

Myxobacteria Bank, Department of Biotechnology, Hoseo University, Asan 336-795, Korea

We prepared culture extracts of 174 Corallococcus and 207 Myxococcus strains isolated in Korea, and compared their antimicrobial activity against Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. The percentage of strains showing antifungal activity was lower in Corallococcus (7.5% [13 of the 174 strains]) than in Myxococcus (51.7% [107 of the 207 strains]). However, the percentage of strains exhibiting antibacterial activity was higher in Corallococcus (12.1% [21 strains]) than in Myxococcus (1% [2 strains]). The culture extracts of 6 Corallococcus strains inhibited both P. aeruginosa and S. aureus and displayed similar high-performance liquid chromatography chromatograms, although the shapes of their fruiting bodies were dissimilar. The rate of production of antibacterial substances was the highest when the strains were cultured in CYS medium for more than 6 days.

Keywords: myxobacteria, antimicrobial activity, Corallococcus, Myxococcus

  1. Belogurov, G. A., M. N. Vassylyeva, A. Sevostyanova, J. R. Appleman, A. X. Xiang, R. Lira, S. E. Webber, S. Klyuyev, E. Nudler, I. Artsimovitch, and D. G. Vassylyev. 2009. Transcription inactivation through local refolding of the RNA polymerase structure. Nature 457: 332-335.
    Pubmed KoreaMed CrossRef
  2. Dawind, W. 2000. Biology and global distribution of myxobacteria in soil. FEMS Microbiol. Rev. 24: 403-427.
  3. Garcia, R., K. Gerth, M. Stadler, I. J. Jr. Dogma, and R. Müller. 2010. Expanded phylogeny of myxobacteria and evidence for cultivation of the 'unculturables'. Mol. Phylogenet. Evol. 57:878-887.
    Pubmed CrossRef
  4. Gerth, K., H. Irschik, H. Reichenbach, and W. Trowitzsch. 1980. Myxothiazol, an antibiotic from Myxococcus fulvus (Myxobacterales). I. Cultivation, isolation, physico-chemical and biological properties. J. Antibiot. 33: 1474-1479.
    Pubmed CrossRef
  5. Gerth, K., S. Pradella, O. Perlova, S. Beyer, and R. Müller. 2003. Myxobacteria: proficient producers of novel natural products with carious biological activities-past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotechnol. 106: 233-253.
    Pubmed CrossRef
  6. Gerth, K., W. Trowitzsch, V. Wray, G. Höfle, H. Irschik, and H. Reichenbach. 1982. Pyrrolnitrin from Myxococcus fulvus (Myxobacterales). J. Antibiot. 35: 1101-1103.
    Pubmed CrossRef
  7. Hagen, D. C., A. P. Bretscher, and D. Kaiser. 1978. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev. Biol. 64: 284-296.
  8. Huntley, S., Y. Zhang, A. Treuner-Lange, S. Kneip, C. W. Sensen, and L. Søgaard-Andersen. 2012. Complete genome sequence of the fruiting myxobacterium Corallococcus coralloides DSM 2259. J. Bacteriol. 194: 3012-3013.
    Pubmed KoreaMed CrossRef
  9. Hyun, H., J. Chung, H. Lee, J. Youn, C. Lee, D. Kim, and K. Cho. 2009. Isolation of cellulose-degrading myxobacteria Sorangium cellulosum. Korean J. Microbiol. 45: 48-53.
  10. Irschik, H. and H. Reichenbach. 1985. The mechanism of action of myxovalargin A, a peptide antibiotic from Myxococcus fulvus. J. Antibiot. 38: 1237-1245.
    Pubmed CrossRef
  11. Irschik, H., K. Gerth, T. Kemmer, H. Steinmetz, and H. Reichenbach. 1983. The myxovalargins, new peptide antibiotics from Myxococcus fulvus (Myxobacterales). I. Cultivation, isolation, and some chemical and biological properties. J. Antibiot. 36: 612.
    Pubmed CrossRef
  12. Irschik, H., R. Jansen, G. Höfle, K. Gerth, and H. Reichenbach. 1985. The corallopyronins, new inhibitors of bacterial RNA synthesis from myxobacteria. J. Antibiot. 38: 145-152.
  13. Kim, Y. S., W. C. Bae, and S. J. Back. 2003. Bioactive substances from myxobacteria. Korean J. Microbiol. Biotechnol. 31: 1-12.
    Pubmed CrossRef
  14. Park, S., B. Lee, J. Kim, C. Lee, E. Jang, and K. Cho. 2004. Isolation and characterization of bacteriolytic wild myxobacteria. Korean J. Microbiol. Biotechnol. 32: 218-223.
  15. Reichenbach, H. 2005. Myxococcales. pp. 1059-1144. In Brenner, D. J., N. R. Krieg, J. T. Staley, and G. M. Garrity (ed.), Bergey's Manual of Systematic Bacteriology, 2nd ed. Bergey's Manual Trust, East Lansing, MI, USA.
  16. Reichenbach, H. and G. Hofle. 1999. Myxobacteria as producers of secondary metabolites. pp. 149-179. In Grabley S. and R. Thiericke (ed.), Drug Discovery from Nature, Springer Verlag, Berlin, Germany.
  17. Reichenbach, H. and M. Dworkin. 1992. The myxobacteria, pp. 3416-3487. In Balows, A., H. G. Trper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The Prokaryotes, 2nd ed., vol. IV, Springer Verlag, New York, USA.
  18. Thierbach, G. and H. Reichenbach. 1981. Myxothiazol, a new antibiotic interfering with respiration. Antimicrob. Agents Chemother. 19: 504-507.
  19. Weissman, K. J. and R. Müller. 2009. A brief tour of myxobacterial secondary metabolism. Bioorg. Med. Chem. 17: 21212136.
    Pubmed KoreaMed CrossRef
  20. Weissman, K. J. and R. Müller. 2010. Myxobacterial secondary metabolites: bioactivities and modes-of-action. Nat. Prod. Rep. 27: 1276-1295.
    Pubmed CrossRef
  21. Wenzel, S. C. and R. Müller. 2009. The impact of genomics on the exploitation of the myxobacterial secondary metabolome. Nat. Prod. Rep. 26: 1385-1407.
    Pubmed CrossRef

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.