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Microbiology and Biotechnology Letters

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Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2013; 41(1): 135-144

https://doi.org/10.4014/kjmb.1211.11004

Received: November 14, 2012; Accepted: January 14, 2013

목형 잎 추출물의 항산화 활성과 멜라닌 생합성에 대한 저해활성

Antioxidative, and Inhibitory Activities on Melanogenesis of Vitex negundo L. Leaf Extract

A Reum Kim 1, Su Ah Park 1, Ji Hoon Ha 1 and Soo Nam Park 1*

서울과학기술대학교 정밀화학과, 화장품종합기술연구소

The aim of this study was to evaluate various aspects of Vitex negundo L. leaf extract, such as the antioxidative activity, tyrosinase inhibitory effects, and inhibitory activities on α-MSH induced melanogenesis, and active component analysis. The DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC50) of the ethyl acetate fraction and aglycone fraction of V. negundo L. leaf extract were 14.51 μg/ml and 13.96 μg/ml, respectively. A luminol-dependent chemiluminescence assay revealed that the reactive oxygen species (ROS) scavenging activity (OSC50) of the aglycone fraction of V. negundo L. leaf extract on ROS generated in an Fe3+-EDTA/H2O2 system was the most prominent at 0.22 μg/ml. The protective effects of the extracts fractions of V. negundo L. leaf against the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1~50 μg/ml). In particular, there were greater protective effects of the aglycone fraction on the cellular membrane than that of the fat-soluble antioxidant (+)-α-tocopherol. The inhibitory effects (IC50) on mushroom tyrosinase were the highest for the ethyl acetate fraction (IC50 = 48.58 μg/ml). The inhibitory effect on α-MSH induced melanogenesis in B16 melanoma cells was 41.80% at 50 μg/ml of ethyl acetate fraction. Active component analyses by TLC, HPLC and LC/ESI-MS revealed luteolin and isoorientin. These results indicate that V. negundo L. leaf extract can be used as an antioxidant for ROS scavenging. Particularly, the luteolin and isoorientin of the ethyl acetate fraction may be applicable to new whitening cosmetics because of its inhibitory effect on mushroom tyrosinase and α-MSH induced melanogenesis in B16 melanoma cells.

Keywords: Vitex negundo, antioxidant activity, antibacterial, tyrosinase, melanogenesis

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