Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2013; 41(1): 105-111

https://doi.org/10.4014/kjmb.1210.10006

Received: October 17, 2012; Accepted: November 15, 2012

생물방제균 Pseudomonas fluorescens 2112의 고추 근권정착능과 Quorum-sensing 기능

Root Colonization and the Quorum Sensing of Antagonistic Bacterium Pseudomonas fluorescens 2112 Involved in the Red-pepper Rhizosphere

Byung-Kwon Jung 1, Yo-Hwan Kim 1 and Sang-Dal Kim 1*

Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea

Biofilm formation of multifunctional plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens 2112 is necessary for P. fluorescens 2112 to have a positive impact on the rhizosphere of red-pepper. This study investigated whether signal molecules of the quorum sensing AHLs are produced in order to confirm biofilm formative ability. Through the use of Petri dish bioassays a blue circle formed evidence of AHLs. It was confirmed that P. fluorescens 2112 produced six-carbon-chain-long AHLs by TLC bioassay. The bacterial density of P. fluorescens 2112 on the top and bottom of pepper plant roots was estimated as 3 × 105 and 8 × 103 CFU/g root, respectively. P. fluorescens 2112 exist more with high-density of 3.5 × 106 CFU/g soil at a depth of 1 cm but at a low-density of 1.1 × 10 CFU/g soil at a depth of 5 cm, from the surface of rhizosphere soil. In addition, biofilm formation of P. fluorescens 2112 on the epidermises and the tips of the red-pepper roots were confirmed visually by SEM. Thus, the production of AHLs by P. fluorescens 2112 brings about quorum sensing signaling and the formation of biofilm on the roots which has a positive effect on economically important crops such as red-pepper by additionally producing a variety of antifungal substances and auxin.

Keywords: Quorum sensing, biofilm, PGPR, biological control, P. fluorescens 2112

  1. Ahmand, J. S. and R. Baker. 1987. Rhizosphere competence of Trichoderma harizianum. Phytopathol. 77: 182-189.
    CrossRef
  2. Brelles-Mariño, G. and E. J. Bedmar. 2001. Detection, purification and characterization of quorum-sensing signal molecules in plant-associated bacteria. J. Biotechnol. 91: 197-209.
    CrossRef
  3. Costerton, J. W., P. S. Stewart, and E. P. Greenberg. 1999. Bacterial biofilms: a common cause of persistent infections. Science 284: 318-322.
    CrossRef
  4. Egamberdiyeva D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl. Soil Ecol. 36: 184-189.
    CrossRef
  5. Fuqua, C., M. R. Parsek, and E. P. Greenberg. 2001. Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing. Annu. Rev. Genet. 35: 439-468.
    Pubmed CrossRef
  6. Khan, A. G. 2005. Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soils in phytoremediation. J. Trace Elem. Med. Biol. 18: 355-364.
    Pubmed CrossRef
  7. Kim, J. W., O. H. Choi, J. H. Kang, C. M. Ryu, M. J. Jeong, J. W. Kim, and C. S. Park. 1998. Tracing of some root colonizing Pseudomonas in the rhizosphere using lux gene introduced bacteria. Kor. J. Plant Pathol. 14: 13-18.
  8. Kim, S. D., L. D. L. Fuente, D. M. Weller, and L. S. Thomashow. 2012. Colonizing ability of Pseudomonas fluorescens 2112, among collections of 2,4-Diacetylphloroglucinol-producing Pseudomonas fluorescens spp. in pea rhizosphere. J. Microbiol. Biotechnol. 22: 763-770.
    Pubmed CrossRef
  9. Lee, E. T. and S. D. Kim. 2000. Selection and actifungal activity of antagonistic bacterium Pseudomonas sp. 2112 against red-pepper rotting Phytophthora capsici. Kor. J. Appl. Microbiol. Biotechnol. 28: 334-340.
  10. Lee, E. T. and S. D. Kim. 2001. An antifungal substance, 2,4Diacetylphloroglucinol, produced from antagonistic bacterium Pseudomonas fluorescens 2112 against phytophthora capsici. Kor. J. Appl. Microbiol. Biotechnol. 29: 37-42.
  11. Lee, E. T., S. K. Lim, D. H. Nam, Y. H. Khang, and S. D. Kim. 2003. Pyoverdin2112 of Pseudomonas fluorescens 2112 inhibits Phytophthora capsici, a red-pepper blight-causing fungus. J. Microbiol. Biotechnol. 13: 415-421.
  12. Lim, J. H., H. Y. Jung, and S. D. Kim. 2009. Development of the microbial corsortium for the environmental friendly agriculture by the antagonistic rhizobacteria. J. Appl. Biol. Chem. 52:116-120.
    CrossRef
  13. Newton, J. A. and R. G. Fray. 2004. Integration of environmental and host-derived signals with quorum sensing during plant-microbe interactions. Cell Microbiol. 6: 213-224.
    Pubmed CrossRef
  14. Park, J. H., I. G. Hwang, J. W. Kim, S. O. Lee, B. Conway, E. P. Greenberg, and K. Lee. 2001. Characterization of quorumsensing signaling molecules produced by Burkholderia cepacia G4. J. Microbiol. Biotechnol. 11: 804-811.
  15. Rad, U. V., I. Klein, P. I. Dobrev, J. Kottova, E. Zazimalova, A. Fekete, A. Hartmann, P. Schmitt-Kopplin, and J. Durner. 2008. Response of Arabidopsis thaliana to N-hexaonyl-DL-homoserine lactone, a bacterial quorum sensing molecule produced in the rhizosphere. Planta 229: 73-85.
    Pubmed CrossRef
  16. Ramey, B. E., M. Koutsoudis, S. B. von Bodman, and C. Fuqua. 2004. Biofilm formation in plant-microbe associations. Curr. Opin. Mircobiol. 7: 602-609.
    Pubmed CrossRef
  17. Rosser, B. T., P. A. Taylor, P. A. Cix, and R. Cluland. 1987. Methods for evaluating antibiotics on bacterial biofilms. Antimicrob. Agents Chemother. 31: 1502-1506.
    CrossRef
  18. Sahu, S. K., K. K. Patnaik, M. Sharmila, and N. Sethunathan. 1990. Degradation of alpha-, beta-, and gamma-hexachlorocyclohexane by soil bacterium under aerobic conditions. Appl. Environ. Microbiol. 56: 3620-3622.
    Pubmed KoreaMed
  19. Sharma, A., M. Sahgal, and B. N. Johri. 2003. Microbial communication in the rhizosphere: Operation of quorum sensing. Curr. Sci. 85: 1164-1172.
  20. Smith, R. S. and B. H. Iglewski. 2003. P. aeruginosa quorumsensing systems and virulence. Curr. Opin. Microbiol. 6: 5660.
    CrossRef
  21. Tsavkelova, E. A., T. A. Cherdyntseva, S. G. Botina, and A. I. Netrusov. 2007. Bacteria associated with orchid roots and microbial production of auxin. Microbiol. Res. 162: 69-76.
    Pubmed CrossRef
  22. Whipps, J. M. 2001. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52: 487-511.
    Pubmed CrossRef
  23. Yoon, S. S. 2006. Quorum sensing mechanism in bacterial communities and their potential applications. Kor. J. Food Sci. Ani. Resour. 26: 402-409.

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.