Microbiol. Biotechnol. Lett. 2011; 39(3): 218-223
호냉성 해양세균 Shewanella sp. L93로부터 Eicosapentaenoic Acid 생산 및 정제를 위한 최적화 조건
Optimal Condition for Eicosapentaenoic Acid Production and Purification from Psychrophillic Marine Baterium Shewanella sp. L93
Sang-Joon Mo , Hye-Won Hong , Ji-Heon Bang and Ki-Woong Cho
Division of Bioscience and Bioinformatics, Myongji University, 1Korea Drug Test Laboratory, 2Department of Marine Biotechnology, Anyang University, 3Department of Marine Biotechnology, Anyang University
Oil pollution was world-wide prevalent treat to the environment, and the physic-chemical remediation technology of the TPH (total petroleum hydrocarbon) contaminated soil had the weakness that its rate was very slow and not economical. Bioremediation of the contaminated soil is a useful method if the concentrations are moderate and non-biological techniques are not economical. The aim of this research is to investigate the influence of additives on TPH degradation in a diesel contaminated soil environment. Six experimental conditions were conduced; (i) diesel contaminated soil, (ii) diesel contaminated soil treated with microbial additives, (iii) diesel contaminated soil treated with microbial additives and the mixture was titrated to the end point of pH 7 with NaOH, (iv) diesel contaminated soil treated with microbial additives and accelerating agents and (v) diesel contaminated soil treated with microbial additives and accelerating agents, and the mixture was titrated to the end point of pH 7 with NaOH. After 10 days, significant TPH degradation (67%) was observed in the DSP-1 soil sample. The removal of TPH in the soil sample where microbial additives were supplemented was 38% higher than the control soil sample during the first ten days. The microbial additives were effective in both the initial removal rate and relative removal efficiency of TPH compared with the control group. However, various environmental factors, such as pH and temperature, also affected the activities of microbes lived in the additives, so the pH calibration of the oil-contaminated soil would help the initial reduction efficiency in the early periods.
Keywords: Bioremediation;denaturing gradient gel electophoresis (DGGE);microbial agents;oil-contaminated soil;
