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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2011; 39(2): 161-167

토양 내 TPH(Total Petroleum Hydrocarbons)의 생물학적 분해 연구

In-situ Bioremediation of Total Petroleum Hydrocarbons-Contaminated Soil by Pseudomonas Species

Jee-Young Kim and Sang-Seob Lee

Department of Life Science, College of Natural Science, Kyonggi University, 1Department of Life Science, College of Natural Science, Kyonggi University

Abstract

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We previously showed that five strains belonging to Pseudomonas could remove TPH (Total Petroleum Hydrocarbons) efficiently when they are applied to TPH-contaminated soil. We optimized the bioremediation condition using different hydrocarbons and nutrients conditions to improve the efficiency. We setup lab-scale column bioreactor to monitor TPH and diesel removal efficiency. When we applied five Pseudomonas sp. mixtures to 25,000 $mg{cdot}kg^{-1}$ TPH-contaminated soil (diesel 10,000 $mg{cdot}kg^{-1}$, kerosene 10,000 $mg{cdot}kg^{-1}$, gasoline 5,000 $mg{cdot}kg^{-1}$) with the optimum condition, 76.3% of TPH removal efficiency was shown for 25 days. Meanwhile, in the application of five Pseudomonas sp. mixtures to 20,000 $mg{cdot}kg^{-1}$ diesel-contaminated soil with the optimum condition, 99.2% of diesel removal efficiency was shown for 40 days. In the application to lab-scale bioreactor with five high efficiency bacteria, 88.5% of TPH removal efficiency was shown for 45 days. Based on the results from this study, we confirmed that this mixed Pseudomonas sp. consortium might improve the bioremediation of TPH in contaminated soil, the efficacy can be controlled by improving the nutrients. We also confirmed that the nutrients and oxygen for biodegradation of TPH could contribute on the management and control of applications of these strains for the study of bioremediation of TPH-contaminated soil.

Keywords: Bioremediation;Pseudomonas sp.;total petroleum hydrocarbons (TPH);bioventing;

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