Microbiol. Biotechnol. Lett. 2011; 39(2): 133-139
천일염 생산공정별 미생물 분포 조사 및 호염미생물 동정
Distribution and Identification of Halophilic Bacteria in Solar Salts Produced during Entire Manufacturing Process
Jong-Min Na , Min-Seung Kang , Jin-Hyo Kim , Yong-Xie Jin , Jeong-Hwan Je , Jung-Bong Kim , Young-Sook Cho , Jae-Hyun Kim and So-Young Kim
Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 1Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 2Chemical Safety Division, National Academy of Agricultural Sciences(NAAS), RDA, 3Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 4Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 5Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 6Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 7Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA, 8Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA
In this study, we determined the changes in microbial numbers in solar salts according to the manufacturing process and storage duration. The salt samples were harvested from salt farms in Shinan (area 2) and Yeonggwang (area 1). They were serially diluted ten-fold and then placed on 4 kinds of cultivable media (mannitol salt agar, eosin methylene blue, plate count agar, and trypticase soy agar). After incubation, we obtained 62 halophilic isolates from the salt samples. Coliform and general bacteria were not detected in all salt samples. By 16S rRNA sequencing analysis, we found 12 kinds of halophilic bacteria belonging to the genera Halobacillus, Halomonas, Bacillus, Idiomarina, Marinobacter, Pseudoalteromonas, Vibrio, Salinivibrio, Virgibacillus, Alteromonas, Staphylococcus and some un-known stains. In our study, we discovered two novel species that have a 16S rDNA sequence similarity below 97%.
Keywords: Halophilic bacteria;mannitol salt agar;solar salt;16S rRNA gene sequencing;
