Production of Bioactive Compounds from Fungi Grown on Ginseng-Steaming Effluent

Jeong-Hoon  Jang; Jae-Ho  Kim; Na-Mi  Kim; Ha-Kun  Kim; Jong-Soo  Lee

Microbiol. Biotechnol. Lett. 2010; 38(2): 129-135

인삼 유출액에서 생육한 곰팡이로부터 생리 활성 물질의 생산

Production of Bioactive Compounds from Fungi Grown on Ginseng-Steaming Effluent

Jeong-Hoon Jang , Jae-Ho Kim , Na-Mi Kim , Ha-Kun Kim and Jong-Soo Lee

Department of Life Science and Genetic Engineering, Paichai University, ¹Korean Food Research Institute, ²Korean Ginseng Corporation, Central Research Institute, ³Department of Life Science and Genetic Engineering, Paichai University, ⁴Department of Life Science and Genetic Engineering, Paichai University

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Abstract

We described production of bioactive compounds from fungi grown on Korean ginseng-steaming effluents (GSE) for develop high-value added nutraceuticals from Korean GSE. Hansenula anomala KCCM 11473, which grew well in Korean GSE had high RNA content, and its optimal autolysis conditions were established to produce 5'-ribonucleotides (13.9~28.5 mg/g of biomass) at $55^{circ}C$ and pH 5.0 for 24 h. 5'-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5'-ribinucleatides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase. Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 mg of S. cerevisiae biomass was produced from 1 g of GSE solid and medicinal ginsenoside-$Rg_3$ contents was determined with 0.033 mg. Mucor miehei KCTC 6011 produced approximately 120 mg of chitosan per g-dry mycelium in 84 h at $25^{circ}C$ when grown in the GSE (pH 8.0) supplemented with 0.5% yeast extract and 0.002% $CuSO_4$. Chitosan produced by M. miehei KCTC 6011 have deacetylated approximately 56% and its viscosity and molecular weight of the chitosan were 80 cps and $1.07 imes10^3$ kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizotonia solani in 60 h.

Keywords: Korean ginseng-steaming effluents (GSE), bioactive compounds, yeasts, filamentous fungi

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eISSN 2234-7305
pISSN 1598-642X

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Jang JH, Kim JH, Kim NM, Kim HK, Lee JS. Production of Bioactive Compounds from Fungi Grown on Ginseng-Steaming Effluent. Microbiol. Biotechnol. Lett 2010;38:129-135.

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인삼 유출액에서 생육한 곰팡이로부터 생리 활성 물질의 생산