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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2009; 37(3): 238-242

Pediococci 선택배지를 이용한 김치 유래 Pediococci 검출

Detection of Pediococci in Kimchi Using Pediococci Selective Medium

Myeong-Jae Lee and Jong-Hoon Lee

Department of Food Science and Biotechnology, Kyonggi University, 1Department of Food Science and Biotechnology, Kyonggi University

Abstract

Pediococci selective medium (PSM) supplemented with ampicillin (A) reported as valid for the detection and enumeration of pediococci included in foods and animal feed was evaluated for the selective detection of the genus Pediococcus in kimchi. PSM is based on the complex basal medium MRS supplemented with cysteine hydrochloride, vancomycin, novobiocin, and nystatin. In the medium evaluation with known species, the growth inhibition of leuconostocs, Pediococcus pentosaceus, Lactobacillus casei, Lactobacillus curvatus, Oenococcus oeni, and Streptococcus thermophilus was not confirmed. In the application of kimchi samples on the selective medium, leuconostocs, P. pentosaceus, Weissella koreensis, Lb. curvatus, Lactobacillus brevis, and Lactobacillus sakei were detected. PSM+A was proved to be not applicable for the detection of pediococci in kimchi.

Keywords: Pediococci selective medium, kimchi, Pediococcus, Leuconostoc, Lactobacillus, Weissella

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