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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2008; 36(4): 360-365

식품 내의 잔류 항생제에 대한 미생물학적 간이검사법의 평가 및 개선

Evaluation and Improvement of Bioassay for Residual Antibiotics in Foods

Min-Hee Park , Tae-Woon Kim , Nam-Uk Jo , Ji-Yoon Jeong , Soon-Ho Lee , Jong-Ok Lee and Hae-Yeong Kim

Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, 1Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, 2Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, 3Pesticide & Veterinary Drug Residues Division, Korea Food and Drug Administration, 4Pesticide & Veterinary Drug Residues Division, Korea Food and Drug Administration, 5Pesticide & Veterinary Drug Residues Division, Korea Food and Drug Administration, 6Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University

Abstract

For the screening of residual antibiotics in foods, bioassays and microbiological inhibitor tests are commonly applied. These methods are tested by the various susceptibility of bacteria against different kinds of antibiotics. However, the sensitivity of bioassay is generally insufficient to detect some residual antibiotics at level of interest. This study was performed to investigate the detection limit of variable antibiotics of the bioassay and to improve the sensitivity to some antibiotics. The sensitivity of bioassay using Bacillus megaterium ATCC 9885, B. subtilis ATCC 6633, B. cereus ATCC 11778 and Geobacillus stearothermophilus ATCC 10149 was low in the detection of macrolides, quinolones, chloramphenicol, and monensin. On the contrary, Micrococcus luteus ATCC 9341 showed high sensitivity to macrolides and Escherichia coli ATCC 11303 was highly sensitive to quinolones and aminoglycosides. Consequently, both strains would be useful to improve sensitivity of bioassay with a wide detection range.

Keywords: Bioassay, antibiotics, screening

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