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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2007; 35(4): 339-346

Bacillus subtilis MJP1이 생산하는 Bacteriocin-Like Substances

Characterization of Bacteriocin-Like Substances Produced by Bacillus subtilis MJP1

Eun-Ju Yang and Hae-Choon Chang

Department of Food and Nutrition, Chosun University, 1Department of Food and Nutrition, Chosun University

Abstract

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The MJP1 bacterial strain, which possesses antifungal activity, was isolated from meju and identified as Bacillus subtilis based on its morphological and biochemical properties, as well as its 16S rRNA sequence. Antimicrobial activity was found against various species of Gram-positive bacteria, yeasts, and molds, including food-spoilage microorganisms. The antifungal activity was found to be stable after heat and proteolytic enzyme treatment, and in the pH range of $6.0{sim}10.0$. The antibacterial activity was stable in the pH range of $6.0{sim}10.0$, but about 50% of the activity was lost after 24 hr at $30^{circ}C$. The antibacterial compound was also inactivated by proteolytic enzyme treatment, indicating its proteinaceous nature. The apparent molecular masses of the partially purified antifungal and antibacterial compounds, as indicated by using the direct detection method in Tricine-SDS-PAGE, were approximately 2.4 kDa and 4.5 kDa, respectively. These studies suggest that B. subtilis MJP1 produces two bacteriocin-like substances with antifungal and antibacterial activities.

Keywords: Bacillus subtilis, bacteriocin-like substance, antifungal activity, antibacterial activity

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