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A flocculating sugar tolerant yeast strain was isolated from fermenting Takju. This strain was identified as Saccharomyces cerevisiae CA-1 according to the Lodder's yeast taxonomic studies. The isolated yeast could grow in 50% glucose and in 7% ethanol in the YPD medium. It's optimal growth temperature, initial pH, shaking rate and initial glucose concentration for ethanol fermentation showed 35$circ$C, 4.5, 150 rpm, 15%, respectively. Ethanol concentration was 63 g/l in 20% glucose after 24 hours, fermentation yield was 0.49 g-ethanol/g-glucose in 10% glucose after 24 hours and ethanol productivity was 3.09 g/l$cdot $h in 10% glucose after 12 hours in batch fermentation. Repeated batch fermentation was possible for over 50 days and ethanol yield, ethanol productivity and substrate conversion rate were 0.39-0.50 g/g, 1.63-2.08 g/l$cdot $h and more than 99%, respectively during these periods.
Keywords: Ethanol fermentation, flocculent Saccharomyces cerevisiae CA-1, screening
JinA Kim and Gwi-Taek JeongMicrobiol. Biotechnol. Lett. 2021; 49(2): 210-216 https://doi.org/10.48022/mbl.2102.02008
Prayolga Toban Palilu , Rina Sri Kasiamdari and Miftahul IlmiMicrobiol. Biotechnol. Lett. 2019; 47(4): 555-564 https://doi.org/10.4014/mbl.1907.07008
Chae Hun Ra and Sung-Koo KimMicrobiol. Biotechnol. Lett. 2016; 44(2): 145-149 https://doi.org/10.4014/mbl.1603.03001