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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1995; 23(6): 723-729

응집성 Saccharomyces cerevisiae CA-1의 분리와 에탄올 발효

Screening and ethanol Fermentation of Flecculent Saccharomyces cerevisiae CA-1

Abstract

A flocculating sugar tolerant yeast strain was isolated from fermenting Takju. This strain was identified as Saccharomyces cerevisiae CA-1 according to the Lodder's yeast taxonomic studies. The isolated yeast could grow in 50% glucose and in 7% ethanol in the YPD medium. It's optimal growth temperature, initial pH, shaking rate and initial glucose concentration for ethanol fermentation showed 35$circ$C, 4.5, 150 rpm, 15%, respectively. Ethanol concentration was 63 g/l in 20% glucose after 24 hours, fermentation yield was 0.49 g-ethanol/g-glucose in 10% glucose after 24 hours and ethanol productivity was 3.09 g/l$cdot $h in 10% glucose after 12 hours in batch fermentation. Repeated batch fermentation was possible for over 50 days and ethanol yield, ethanol productivity and substrate conversion rate were 0.39-0.50 g/g, 1.63-2.08 g/l$cdot $h and more than 99%, respectively during these periods.

Keywords: Ethanol fermentation, flocculent Saccharomyces cerevisiae CA-1, screening

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