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The effect of AcNPV infection conditions such as serum concentration, pH, CaCl2, lysosomotropic agent, cell density at infection, agitation, aeration and nutritional supplementattion on recombinant protein production in Spodoptera frugiperda 21 cells was investigated using tissue culture flask, bottle and spinner flask. It was shown that serum, CaCl2, pH and cell density at infection affected recombinant production. The lysosomotropic agent did not significantly influence recombinant protein production.
Keywords: Recombinant protein production, Spodoptera frugiperda 21, Autographa californica nuclear polyedrosis virus, infection
Eun Ju Oh, Joowon Park, and Jae Kyung Kim*Microbiol. Biotechnol. Lett. 2021; 49(2): 249-254 https://doi.org/10.48022/mbl.2010.10014
Jae-Sik Jeon and Jae Kyung KimMicrobiol. Biotechnol. Lett. 2018; 46(4): 425-429 https://doi.org/10.4014/mbl.1805.05006