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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1993; 21(5): 504-510

AcNPV 감염 조건이 Spodoptera frugiperda 21 세포에서의 재조합 단백질 생산에 미치는 영향

Effect of AcNPV Infection Conditions on Recombinant Protein Production in Spodoptera frugiperda 21 Cells

Abstract

The effect of AcNPV infection conditions such as serum concentration, pH, CaCl2, lysosomotropic agent, cell density at infection, agitation, aeration and nutritional supplementattion on recombinant protein production in Spodoptera frugiperda 21 cells was investigated using tissue culture flask, bottle and spinner flask. It was shown that serum, CaCl2, pH and cell density at infection affected recombinant production. The lysosomotropic agent did not significantly influence recombinant protein production.

Keywords: Recombinant protein production, Spodoptera frugiperda 21, Autographa californica nuclear polyedrosis virus, infection

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