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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1986; 14(6): 433-439

동물세포내에서의 유체성 세균의 증식

Amplification of Chlamydia trachomatis in Animal Cell Host

Guhn-Been Yim , Cha-Yong Park and Suk-Il Hong

Department of Chemical Technology College of Engineering Seoul National University, 1Department of Chemical Technology College of Engineering Seoul National University, 2Yong Nam University, Medical School

Abstract

Abundant amount of Chiamydia trachomatis could be amplified in mammalian McCoy cells and purified using descontinuous Uroarafin gradient centrifugation. As a chemical means io increase the Chlamydia trachomatis inclusions in McCoy cells IUdR treatment was found to be more effective than the cycloheximide treatment and was recommendanble for the proliferation of Chlamydia trachomatis. Centrifugation promoted Chlamydia trachomatis adhesion to McCoy cell surface, and maximal percentage of infected cells was obtained at about 3000g. The purified Chlamydia trachomatis could be kept in SPG solution for 48 hours at +4$^{circ}C$ but for longer storage freezing to -7$0^{circ}C$ was necessary.

Keywords: Chlamydia trachomatis, inclusion body, McCoy cell

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