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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1986; 14(1): 97-101

Bacillus thuringiensis 살충제의 개발에 관한 연구 2. B. thuringiensis subspp. 내독소 단백질의 전기영동 분석

Studies on the Development of the Bacillus thuringiensis pesticide 2. Electrophoretic analysis of B. thuringiensis subspp. endotoxin

Hyeong-Hoan Lee , Tae-Sook Kang and Jung-Hee Seo

Inst. for Genetic Engineering, Dept. of Biology, Kon Kuk Univ., 1Inst. for Genetic Engineering, Dept. of Biology, Kon Kuk Univ., 2Inst. for Genetic Engineering, Dept. of Biology, Kon Kuk Univ.

Abstract

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Alkali-solubilized endotoxin proteins of B. thuringiensis subsp. were purified by Sepharose gel filtration and then aliquotes of the fractions showing peaks were dialyzed and electrophoresed on SDS-polyacrylamide gel. Each subspecies showed two protein bands on the gel. The subunit protein molecular weights of Bt. var. kurstaki were approximately 138,000 and 69,000 daltons, those of Bt var. thuringiensis 132,000 and 70,000, Bt var. sotto 133,000 and 71,000, Bt. var. pakistani 134,000 and 72,000, Bt. var. israelensis 128,000 and 71,000 and Bt. var. tolworthi 135,000 and 69,000.

Keywords: B. thuringiensis, endotoxin

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