Genome Report | Genome Report
Microbiol. Biotechnol. Lett. 2024; 52(1): 102-104
https://doi.org/10.48022/mbl.2401.01002
Sihyun An, Gyeongjun Cho, Jae-Hyung Ahn, Hang-Yeon Weon, Dayeon Kim, Young-Joon Ko, Jehyeong Yeon, Joon-hui Chung, Han Suk Choi, and Jun Heo*
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea
Correspondence to :
Jun Heo , bioheojun@korea.kr
Massilia sp. KACC 81254BP, isolated from a landfill on Jeju Island, Republic of Korea, possesses the capability to degrade polyhydroxyalkanoates (PHAs). The genomic analysis of strain KACC 81254BP consists of a circular chromosome comprising 5,028,452 base pairs with a DNA G+C content of 64.6%. This complete genome consists of a total of 4,513 genes, including those encoding the PHA degradation enzyme (PhaZ). This study offers valuable genomic insights into the enzymes responsible for degrading polyester plastics.
Keywords: Bacteria, Massilia, polyhydroxyalkanoate degradation, genome
Polyhydroxyalkanoates (PHAs) are known to be biodegradable plastics that can replace conventional petrochemical- derived plastics [1], but there is still limited information on the bacteria and their enzymes that degrade PHAs. To date, it is known that manufactured PHA can be degraded by microorganisms, such as
For the identification of strain at the species level, the orthologous average nucleotide identity (OrthoANI) value between
The amino acid sequences and reported PHA-related enzyme sequences [9] were aligned and compared by sword (version 1.0.4). In the comparison, genes analyzed to decompose plastic were searched based on identification of more than 50%. The PHA-related cluster genes obtained from Prokka were visualized on the genome map by ggplot2 (version 3.4.3) in R (version 4.1.3) (Fig. 1). We identified six genes related to the PHA-related cluster including
Table 1 . PHA-related cluster characteristics of the
PHA-related cluster* | Protein (name) | Locus tag | Similarity (%) |
---|---|---|---|
acetyl-CoA C-acetyltransferase | Q4S45_19985 | 74 | |
acetoacetyl-CoA reductase | Q4S45_19980 | 61 | |
acetoacetyl-CoA reductase | Q4S45_04890 | 52 | |
class I poly(R)-hydroxyalkanoic acid synthase | Q4S45_07650 | 60 | |
- | - | - | |
- | - | - | |
polyhydroxyalkanoate synthesis repressor PhaR | Q4S45_07655 | 75 | |
Polyhydroxyalkanoate depolymerase | Q4S45_02195 | 65 |
*PHA-related gene and protein sequences of the model organism Cupriavidus necator H16 (AM260479) [9].
The complete genome sequence of
제주도 매립지에서 분리된
This study (Project No. RS-2020-RD009785) was carried out with the support of National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.
The authors have no financial conflicts of interest to declare.
Du-Gyeong Han, Ji-A Jeong, Sung-Kyoung Lee, Seong-Han Kim, and Se-Mi Jeon
Microbiol. Biotechnol. Lett. 2024; 52(2): 211-214 https://doi.org/10.48022/mbl.2403.03005Jihye Jung, Dawon Jo, Myoungjoo Riu, Seongho Ahn, and Do-Hyun Kim
Microbiol. Biotechnol. Lett. 2024; 52(2): 208-210 https://doi.org/10.48022/mbl.2403.03009Do-Won Jeong
Microbiol. Biotechnol. Lett. 2024; 52(1): 91-93 https://doi.org/10.48022/mbl.2402.02008