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Microbiology and Biotechnology Letters


Molecular and Cellular Microbiology  |  Microbiome

Microbiol. Biotechnol. Lett.

Received: August 16, 2021; Revised: September 8, 2021

Determination of semen quality and antibacterial susceptibility pattern of bacteria isolated from semen of Iraqi subjects

Anwer Jaber Faisal and Hamzah Abdulrahman Salman *

1Al-Farabi University College, 2The University of Mashreq

Correspondence to :
Hamzah Abdulrahman Salman, Department of Medical Laboratory Techniques, College of Medical Sciences Techniques, The University of Mashreq, Baghdad, Iraq. Fax : +9647732914480, E-mail :


Infertility is one of the main problems that affect men's mood and behaviour. Microorganisms are one of the primary etiological agents that may be associated with infertility. The objective of the present study was to identify the bacterial causative agents from semen of infertile subjects and determine the influence of bacterial infection on sperm quality, as well as determine the antibacterial susceptibility testing. Forty semen samples (30 infertile patients and 10 fertile) were taken. The pH, volume, motility, and concentration of semen were analyzed. The samples were processed and identified based on biochemical tests using API identification kits. The antibiotics susceptibility pattern was determined using the disc diffusion method. Abnormal sperm quality was observed. The mean averages of age, sperm morphology, concentration, progressive motility, pH level and pus cell were 31.9 years, 2.7 %, 10.4 million/ml, 27.3 %, 8.3 and 5.7, respectively. Among the tested sperm, oligoasthenozoospermia was the highest 27/30, followed by teratozoospermia 25/30 and asthenozoospermia 22/30. Of the tested infertile patients' sperm, 19, 6, and 5 were identified as E. coli, K. pneumoniae and S. epidermidis, respectively. The results revealed multi-drug resistant bacteria. Among the tested antibiotics, amikacin showed higher activity against all isolated bacteria. However, gentamicin, cefotaxime, levofloxacin and ampicillin exhibited maximum resistance. Leukocytospermia and bacterial infections are possibly responsible for sperm abnormality. Multi-drug resistant bacteria were detected. Amikacin was the most effective antimicrobial agent against the isolated bacteria. While gentamicin, cefotaxime, levofloxacin and ampicillin were shown highest resistance.

Keywords: Infertility, Antibiotics, Multi-drug resistant,, Amikacin, Leukocytospermia, E. coli

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