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Microbiology and Biotechnology Letters

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Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2013; 41(3): 350-357

https://doi.org/10.4014/kjmb.1303.03004

Received: March 13, 2013; Accepted: August 14, 2013

북극 지의류 Stereocaulon spp로부터 분리한 여러 미생물의 항산화 성질

Antioxidant Properties of Various Microorganisms Isolated from Arctic Lichen Stereocaulon spp.

Mi-Kyeong Kim 1, Hyun Park 2 and Tae-Jin Oh 1*

1Department of Pharmaceutical Engineering, SunMoon University, Asansi, Chungnam 336-708, Korea, 2Korea Polar Research Institute (KOPRI), Songdo TechnoPark, Yeonsu-gu, Incheon 406-840, Korea

Abstract

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Lichens are symbiotic organisms composed of fungi, algae, or cyanobacteria which are able to survive in extreme environmental conditions ranging from deserts to polar areas. Some lichen symbionts produce a wide range of secondary metabolites that have many biological activities such as antibacterial, antifungal, antiviral, antitumor, antioxidant and anti-inflammatory etc. Among the symbionts of lichens, of the bacterial communities of lichen symbionts little is known. In this study, we isolated 4 microbial species from the Arctic lichen Stereocaulon spp. and evaluated their antioxidant properties using 1,1-diphenyl-2-picryl- hydrazyl assay as well as 2,2'-azino-bis(3-ethyl benzothiazoline-6-sulphonic acid) assay. Total phenolic contents and total flavonoid contents were also measured. A potent radical scavenging activity was detected in a number of the lichen extracts. Among the 4 species tested in this study, the ethyl acetate extract of Bosea vestrisii 36546(T) exhibited the strongest free radical scavenging activity, with an inhibition rate of 86.8% in DPPH and 75.2% in ABTS assays. Overall, these results suggest that lichen-bacteria could be a potential source of natural antioxidants.

Keywords: ABTS, antioxidant property, Arctic lichen, DPPH, Stereocaulon spp, TPC

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