Purification and Properties of Alkaline Lipase from Pseudomonas sp. J-19

Microbiol. Biotechnol. Lett. 1991; 19(1): 57-63

Pseudomonas sp. J-19가 생산하는 Alkaline Lipase의 정제와 특성

Purification and Properties of Alkaline Lipase from Pseudomonas sp. J-19

Abstract

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Abstract

A strain J-19 was isolated from soil, produced lipase which has resistant against alkali and linear alkylbenzene sulfonate. The strain was identified as Pseudornonns sp.. The enzyme was purified by ammonium sulfate precipitation, DEAE-Sephadex and Sephadex G- 100 column chromatography. The specific activity of the purified enzyme was 35 unit/mg protein and the yield of enzyme activity was 17%. The purified enzyme showed a single band on polyacrylamide disc gel electrophoresis. Mo1ecul;tr weight of the purified enzyme was estimated about 36,000 by Sephadex GI00 gel filtration and SDS-polyacrylarnide gel electrophoresis. The optimum pH and temperature were pH 10.0 and $30^{circ}C$, respectively. Activity of the purified enzyme was increased 2-fold by the addition of 0.1% linear alkylbenzene sulfonate and 2.5- fold by the addition of 0.05% Tide. This enzyme remained stable from pH 8.0 to 10.0 and stable up to $40^{circ}C$.

Keywords: Pseudomonas, alkaline lipase, linear alkylbenzene sulfonate resistant lipase