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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1990; 18(4): 394-400

유전자 재조합 대장균 발효의 최대 생산성을 위한 생육에서 제품 생성으로 전환시기의 최적화

Optimization of Switching Time from Growth to Product Formation for Maximum Productivity of Recombinant Escherichia coli Fermentation

Y. Patkar Anant

Abstract

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Maximization of productivity of recombinant cell fermentations requires consideration of the inverse relationship between the host cell growth rate and product formation rate. The problem of maximizing a weighted performance index was solved by using optimal control theory for recombinant E. coli fermentation. Concentration of a growth inhibitor was used as a control variable to manipulate the specific growth rate, and consequently the cloned-gene expression rate. Using a simple unstructured model to describe the main characteristics of this system, theoretical analysis showed that the optimal control profile results in an initial high growth rate phase followed by a low growth rate and high product formation rate phase. Numerical calculations were done to determine optimal switching times from the growth to the production stage for two representative cases corresponding to different dependency of the product formation rate on the growth rate. For the case when product formation rate is sensitive to the specific growth rate, the optimized operation yields about 60% increase in the final product concentration compared with a simple batch fermentation.

Keywords: Recombinant E.coil, optimization, fermentation

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