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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1990; 18(2): 126-131

Clostridium 속 세균포자의 안정성과 Plasmids 탐색

Stability of Clostridial Spore in Soil Culture and Screening for Plasmids in the Clostridium strains

Ki-Hong Yoon , Jung-Kee Lee , Byung-Hong Kim , Sun-Joo So , Yung-Sook Chun , Gi-Seok Kwon and Z.G. Zeikus

Applied Microbiol. Lab., Genetic Engineering Center, Korea Institute of Science and Technology, 1Applied Microbiol. Lab., Genetic Engineering Center, Korea Institute of Science and Technology, 2Applied Microbiol. Lab., Genetic Engineering Center, Korea Institute of Science and Technology, 3Applied Microbiol. Lab., Genetic Engineering Center, Korea Institute of Science and Technology, 4Applied Microbiol. Lab., Genetic Engineering Center, Korea Institute of Science and Technology, 5Applied Microbiol. Lab., Genetic Engineering Center, Korea Institute of Science and Technology, 6Michigan Biotechnology Institute

Abstract

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Stock cultures of Clostridim, which had been maintained as spores with soil as carrier for 50-70 years, were used to determine their revival ratio, and plasmids were screened among the revived strains. From 131 soil cultures, 100 strains grew in the liguid medium giving the revival ratio of 76%. Analyses of the fermentation products divided the revived strains into 63 solventogenic strains and 37 acidogenic strains. Agarose gel electrophoresis showed one or more plasmid bands in the cleared lysates of 50 revived cultures. The sizes of the plasmids range in size from 1 to more than 50 kilobase pairs.

Keywords: Clostridium sp., plasmid, clostridial spore

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