Effects nit Mineral Salts on the Improvements of Sisomicin field

Chul-S  Shin; H. Han  Sang; Sang H.  Lee

Microbiol. Biotechnol. Lett. 1989; 17(3): 247-251

무기질 염이 Sisomicin 발효 수율의 증가에 미치는 영향

Effects nit Mineral Salts on the Improvements of Sisomicin field

Chul-S Shin , H. Han Sang and Sang H. Lee

Department of Food Engineering, Yonsei University, ¹Department of Food Engineering, Yonsei University, ²Korea Research Institute of Chemical Technology

Abstract

Go to

Abstract

Effects of mineral salts on sisomicin fermentation were investigated. The optimal concentration of CoCl$_2$for accomplishing a high antibiotic yield was found to be 16.8 $mu$M at which it could function as a cofactor. At this level the other mineral salts tested had no effect. On the other hand, at much higher concentration levels (above 1 mM), four mineral salts such as ZnSO$_4$, KH$_2$PO$_4$, FeSO$_4$and MgSO$_4$were used in order to liberate the intracellular sisomicin out-side the cells, because the sisomicin accumulated mostly in cells and it was supposed to limit the improvement of antibiotic yield. ZnSO$_4$and KH$_2$PO$_4$had no effect at all, and FeSO$_4$brought about some improvement. However, by keeping the concentration of MgSO$_4$to be 25 mM or higher in culture broths, the antibiotic yield could be improved by more than 100%, partially due to the enhanced liberation of the intracellular antibiotic.

Keywords: Sisomicin, fermentation, Mineral Salts

Article Tools

Share this article on :

Most Searched Keywords ?
What is Most Searched Keywords?

It is most registrated keyword in articles at this journal during for 2 years.

eISSN 2234-7305
pISSN 1598-642X

Download to citation(s)

Shin CS, Sang HH, Lee SH. Effects nit Mineral Salts on the Improvements of Sisomicin field. Microbiol. Biotechnol. Lett 1989;17:247-251.

Download to citation(s)
Export type	* Please select a format and type blew.
Format	EndNote Reference Manager RIS Format Plain Text

무기질 염이 Sisomicin 발효 수율의 증가에 미치는 영향