Conjugal Transfer of NAH, TOL, and CAM::TOL＊ Plasmid into n-Alkane Assimilating Pseudomonas putida

Yung-Hee  Kho; Hyo-Kon  Chun; Kyong-Yun  Cho; Kyung-Sook  Bae

Microbiol. Biotechnol. Lett. 1989; 17(1): 51-55

방향족 탄화수소 분해 Plasmid의 n-Alkane 자화성 Pseudomonas putida에로의 전이

Conjugal Transfer of NAH, TOL, and CAM::TOL＊ Plasmid into n-Alkane Assimilating Pseudomonas putida

Yung-Hee Kho , Hyo-Kon Chun , Kyong-Yun Cho and Kyung-Sook Bae

Genetic Engineering Center, KAIST, ¹Genetic Engineering Center, KAIST, ²Genetic Engineering Center, KAIST

Abstract

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Abstract

The conjugally transferred TOL plasmid or NAH plasmid was stably maintained and expressed in n-alkane assimilating Pseudomonas putida KCTC 2405. However, these plasmids were not able to coexist in this strain because of incompatibility. The incompatibility of TOL and NAH plasmid was bypassed using CAM::TOL* plasmid, which was constructed by the transposition of only tol gene without incompatibility system in TOL plasmid into CAM plasmid. p. putida 3SK capable of growing on m-toluate, naphthalene, camphor, and n-alkane(C8-C24) was constructed by the conjugal transfer of NAH plasmid into n-alkane assimilating p. putida SK carrying CAM:: TOL* plasmid. CAM::TOL＊ plasmid in p. putida 3SK was stable on the selective media but unstable on the nonselective media.

Keywords: Conjugal Transfer, n-Alkane Assimilation, Pseudomonas putida, plasmid

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Kho YH, Chun HK, Cho KY, Bae KS. Conjugal Transfer of NAH, TOL, and CAM::TOL＊ Plasmid into n-Alkane Assimilating Pseudomonas putida. Microbiol. Biotechnol. Lett 1989;17:51-55.

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방향족 탄화수소 분해 Plasmid의 n-Alkane 자화성 Pseudomonas putida에로의 전이