Production of $alpha$-Amylase using Aqueous Two-Phase System

J.S.  Choi; Yoo  Y.J.

Microbiol. Biotechnol. Lett. 1988; 16(5): 358-362

수성 2상계를 이용한 알파-아밀라제의 생산

Production of $alpha$-Amylase using Aqueous Two-Phase System

J.S. Choi and Yoo Y.J.

Biochemical Engineering tab. Department of Chemical Engineering Seoul National University, ¹Biochemical Engineering tab. Department of Chemical Engineering Seoul National University

Abstract

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Abstract

Aqueous two-phase fermentation system was tested for the overproduction of extracellular enzyme through $alpha$-amylase fermentation by Bacillus amyloliquefaciens. By employing aqueous two-phase system $alpha$-amylase activity showed 25% increase compared to the result using regular medium and no deactivation of the enzyme was observed. The presence of polyethylene glycol was observed to promote the enzyme production, while to inhibit the growth of the microorganism. It is recommended that polyethylene glycol be added during the log-growth phase and dextran be added after the enzyme activity reaches Its maximum for efficient $alpha$-amylase fermentation and in situ recovery of the enzyme.

Keywords: Aqueous Two-Phase, Bacillus amyloliquefaciens, $alpha$-amylase, fermentation, enzyme deactivation

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eISSN 2234-7305
pISSN 1598-642X

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Choi J, Y.j. Y. Production of $alpha$-Amylase using Aqueous Two-Phase System. Microbiol. Biotechnol. Lett 1988;16:358-362.

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수성 2상계를 이용한 알파-아밀라제의 생산