보문(Article)
Fermentation and Bioprocess Engineering
Microbiol. Biotechnol. Lett. 2015; 43(4): 343-349
https://doi.org/10.4014/mbl.1510.10003
Received: October 12, 2015; Accepted: November 6, 2015
표면적이 증가된 분별침전에서 친수성 고분자물질을 이용한 paclitaxel의 입자 크기 감소
Decrease in the Particle Size of Paclitaxel by Increased Surface Area Fractional Precipitation Process Using Hydrophilic Polymer
Yong Kyu Lim 1 and Jin-Hyun Kim 1*
Department of Chemical Engineering, Kongju National University, Cheonan 331-717, Republic of Korea
In this study, we applied an increased surface area fractional precipitation with hydrophilic polymer for decreasing the particle size of the anticancer agent paclitaxel from plant cell cultures. The addition of polymer resulted in a considerable decrease in the size of the paclitaxel precipitate. Among the polymers (HPMC 2910, PVP-K90, PVA) used, PVP-K90 was the most effective for the inhibition of precipitate growth. When PVP-K90 (0.2%, w/v) was used, the paclitaxel particles were four to five times smaller, having less than a 20 μm radius, than those obtained in the absence of the polymer. In addition, the precipitate size was inversely correlated with the absolute value of zeta potential.
Keywords: Pacitaxel, Increased surface area fractional precipitation, Polymer, Particle size, Decrease
